Purpose: The purpose of this study was aimed to investigate and analyze the electromyographic (EMG) difference and kinetics of lower extremity for volleyball players during continuous blocking jumps. Methods: Twelve Division I collegiate volleyball players (height: 185.5 ± 3.5 cm, weight: 79.5 ± 5.2 kg, age: 20.5 ± 1.5 years) were recruited in this study. The participants were required to perform 14 blocking jump with 90% maximum jump height for 3 sets. The rest time between sets is three minutes. Ten Vicon T40 motion analysis system, one Kistler force-plant and BTS Free EMG were used to collect the kinematic, kinetic and EMG signal variables. All the data were processed by a self-development program. The peak power output (PPO), maximum rate of force development (mRFD) and relative net impulse (RNI) were calculated by kinetic parameter form each jump. The mean frequency (MF) of rectus femoris muscle (RFM), biceps femoris muscle (BFM), anterior tibialis muscle (ATM) and gastrocnemius muscle (GM) were computed form EMG signal. Results: The results of this study showed that the continuous blocking jump caused the MF of the RFM to decrease by 3.0% and 6.1%, respectively, and the MF of the ATM decreased by 3.5% and 6.7%, respectively. In addition, the mRFD within 30 milliseconds also decreased by 3.5%. Conclusion: Under the fatigue condition of repeated jumping, the volleyball player's performance of vertical jump was affected by the fatigue of the knee extensor muscle, so it can not produce the best muscle strength, maximum power output and coordination ability.