The purpose of this research is to explore the impact of different stretching modes on the kick action in Taekwondo. The research participants were nine male students with black belt level one from the R.O.C. Military Academy. The repeated measure design was adopted in this research. The participants first underwent a 6-minute jogging warm-up and a 2-minute joint exercise. After performing different stretching modes (non-stretching, static stretching, and dynamic stretching), roundhouse kick and slide roundhouse kick movement testing. The kick time, average of EMG amplitude (%MVC), and other data were processed using the SPSS for Windows 18.0 Version software. Independent sample t test and one-way ANOVA were carried out. If the significant standard of p＜.0.5 was reached, the LSD was conducted for post hoc comparisons. Findings show that in the roundhouse kick start stage and in terms of the overall movement time, dynamic stretching was significantly superior to dynamic stretching; in the slide roundhouse kick start time stage, dynamic stretching was significantly superior to static stretching. In addition, in the part of average of EMG amplitude (%MVC) performance, in the roundhouse kick start stage, the biceps in dynamic stretching was significantly superior to that of dynamic stretching and non-stretching modes; the medial gastrocnemius in static stretching was significantly superior to that of the non-stretching mode; the lateral gastrocnemius in static stretching was significantly superior to that of the non-stretching mode. In the roundhouse kick stage, the rectus femoris in dynamic stretching was superior to that of the non-stretching mode. In the overall roundhouse kick movement, the rectus femoris was significantly superior to that of the non-stretching mode. In static stretching, the lateral gastrocnemius was superior to that of the dynamic stretching and non-stretching modes. In the slide roundhouse kick start stage, the rectus femoris in dynamic stretching was significantly superior to that of static stretching and without stretching modes. In the slide and roundhouse kick stage, the semitendinosus was significantly superior to that of the static stretching mode. As for the overall slide roundhouse kick movement, the rectus femoris in dynamic stretching was significantly superior to that of the static stretching and non-stretching modes; the semitendinosus in dynamic stretching was significantly superior to that of static stretching.