MOESM3 of Enhancing the toolbox to study IL-17A in cattle and sheep

Additional file 3. Summary of commercial IL-17A antibody evaluation for capacity to bind intracellular recombinant bovine and ovine IL-17A in fixed Chinese Hamster Ovary cells. Control antibodies are listed in lower case, the anti-IL-17A antibodies are listed in upper case. Using the gating strategy described in brief in evaluation of commercial antibodies subsection (displayed in Additional file 2) the following data are shown: P1/P2/P3/P4 percentage of cells above the region boundary line of phycoerythrin or alexafluor 488 channels vs Side Scatter-Area (denoted #%); P1/P2/P3 median fluorescence region value (phycoerythrin or alexafluor 488 channels); and delta median fluorescence intensity (deltaMFI) calculated by taking the median fluorescence region values (phycoerythrin or alexafluor 488 channels) for the commercial IL-17A antibody and deducting the median fluorescence region value of the appropriate isotype or equivalent control antibody in the same fluorochrome channel) for the CHO UTF, bovIL-17A and ovIL-17A transfected, fixed CHO cells.