Additional file 1: Figure S1. of Analysis of 5’ gene regions reveals extraordinary conservation of novel non-coding sequences in a wide range of animals

Association of CNEs with predicted nucleosome occupancy. Figure S2. Relationship of transcription factor binding site motifs with GC content. Figure S3. CNE sequences show an underrepresentation of upstream ATG trinucleotides. Figure S4. Ferritin 5’ UTR iron response element (IRE) alignment diagram. Figure S5. Conserved CNE is a novel hairpin in the 5’ UTR of the Paramyosin gene. Figure S6. Uncharacterized Osiris gene cluster contains several CNEs. Conserved upstream sequence location relative to the coding sequences and combined conservation scores of six genes in the Osiris cluster. Figure S7. Three CNEs in the 5’ UTR of UNR. Conserved upstream sequences located in the 5’ UTR of the master regulator UNR. Figure S8. Association of CNEs with GC content. Average GC content across the genomic regions containing the CNEs, compared to control sets. Figure S9. A schematic diagram of the alignment strategy used in this study. Figure S10. A schematic diagram of the bundling strategy. Three pairwise CNEs found conserved between N. vitripennis and two other species are bundled into a single CNE. Figure S11. Comparison of ortholog identification methods. (PDF 340 kb)