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Additional file 2 of Dietary-derived vitamin B12 protects Caenorhabditis elegans from thiol-reducing agents

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posted on 2022-10-10, 03:15 authored by Alan D. Winter, Elissa Tjahjono, Leonardo J. Beltrán, Iain L. Johnstone, Neil J. Bulleid, Antony P. Page
Additional file 2: Additional Materials and Methods. gDNA isolation, Sanger sequencing confirmations of rips-1 alleles in DTT strains, total RNA extraction, 3′RACE for rips-1, and genotyping. Additional Results. 3′ untranslated region from rips-1 (R08E5.3). Table S1. DTT resistance alleles. Table S2. RNAi mini-screen for B12-related pathways. Table S3.C. elegans strains. Table S4. Oligonucleotide primers. Table S5. Plasmids. Additional References.

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Biotechnology and Biological Sciences Research Council

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    BMC Biology

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    Vitamin B12Reductive stressDTTMethyltransferaseMethionine

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