Additional file 15 of Bivalent promoter hypermethylation in cancer is linked to the H327me3/H3K4me3 ratio in embryonic stem cells

Figure S13. Absence of hypermethylation at H4Kme3-only and H3K27me3-only promoters. To test if cancer-specific DNA hypermethylation was more associated with high ratio H3K27me3:H3K4me3 loci (hiBiv) than singly marked promoters, we analysed promoters characterised by the enrichment of either K4me3-only or H3K27me3-only in human ES cells. We repeated our approach detailed in Fig. 6f and found little evidence of promoter hypermethylation in either breast or colon tumours at these loci.