200 μg mitochondria were incubated in respiration buffer at 25°C and oxygen consumption was monitored in the presence of 5 mM succinate

(A) RCRs, (B) state III respiratory rates in the presence of 50 μM ADP, (C) uncoupled respiratory rates in the presence of 10 μM CCCP, (D) percent inhibition by 40 μM atractyloside compared with state III rates (mean ± SEM). (E) ADP:O ratios in the presence of 2 mM NADH, (F) ATP production (percentage of wt) by complex V in the presence of 5 mM NADH, 2 mM ADP, and 10 μM adenylate kinase inhibitor, ApA (mean ± SEM). Aliquots taken at 30, 60, 90, 120, and 150 s after addition of 2 mM ADP were extracted with PCA and ATP levels measured. Unless indicated by “NS”, all differences compared with wt are statistically significant (P ≤ 0.05) by test. = 6–11 (A–D), = 5–9 (E), and = 4 (F).

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Taken from "Cardiolipin defines the interactome of the major ADP/ATP carrier protein of the mitochondrial inner membrane"

The Journal of Cell Biology 2008;182(5):937-950.

Published online 8 Sep 2008

PMCID:PMC2528576.