Effect of the expression level of the fused proteins according to the identity of the second codon

Copyright information:

Taken from "Use of signal sequences as an removable sequence element to stimulate protein synthesis in cell-free extracts"

Nucleic Acids Research 2006;35(4):e21-e21.

Published online 20 Dec 2006

PMCID:PMC1849898.

© 2006 The Author(s)

The efficiency of cell-free protein synthesis was enhanced by switching the second codon of ss and ss into AAA (). In contrast, protein synthesis was repressed when the second codons (AAA) of ss and ss were mutated into NGG codons (AGG, TGG, GGG and CGG) (). After 2 h incubation, 15 μl of the reaction samples were withdrawn from the reaction mixture, and the [C]leucine-labeled radioactivity was measured as described in Materials and Methods.