Epigenetic Control of the foxp3 Locus in Regulatory T Cells
Figure 4
Increased Histone Acetylation and K4 Trimethylation in CD25+CD4+ Tregs
ChIP assays were performed with CD25+CD4+ Tregs (filled bars) and conventional CD25−CD4+ T cells (open bars) sorted from spleens and LNs pooled from 20 male BALB/c mice. DNA fragments binding to acetylated or trimethylated histones were immunoprecipitated using antibodies directed against acetylated histone H3, acetylated histone H4, or trimethylated histone H3 at position K4. A rabbit isotype immunoglobulin G (IgG) served as control. Precipitated DNA was quantified by real-time PCR with primers specific for the differentially methylated region of the foxp3 locus. Sample PCR products were set in relation to input DNA. One representative experiment out of two individual experiments is shown.