PXD039886

PXD039886 is an original dataset announced via ProteomeXchange.

Title	Distinct gain of function mechanism for the hot-spot mutant p53R175H
Description	TP53 (p53) is the most commonly mutated gene in human cancers, and p53 missense mutations are present in more than 40% of all human tumors. Most p53 mutations are located within the DNA binding domain, including hotspot mutations R175H, R248W, and R273H. To elucidate the precise mechanism by which p53 missense mutants execute their gain-of-functions (GOFs) in vivo, we used a proteomic screen to identify any protein that recognizes the p53 DNA-binding domain (DBD) in a manner dependent on its mutation status. To do so, we first purified the potential binding proteins associated with the p53 DBD through multi-step affinity chromatography from a SFB-p53 H1299 stable cell line. The affinity-purified SFB-p53 interacting proteins were detected by liquid chromatography mass spectrometry/mass spectrometry (LC–MS/MS) and revealed a few cellular proteins that have been reported to interact with the DNA binding domain of p53, such as TP53BP1, USP28, and Sirt1. Interestingly, we also identified many new interacting proteins from the same complex.
HostingRepository	PRIDE
AnnounceDate	2023-02-24
AnnouncementXML	Submission_2023-02-24_01:50:33.994.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039886
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	huanli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-06 05:45:18	ID requested
1	2023-02-17 03:52:49	announced
⏵ 2	2023-02-24 01:50:34	announced	2023-02-24: Updated project metadata.

10.6019/PXD039886;

submitter keyword: p53R175H, GOF, LSD2, ferroptosis, BACH1, oxidative stress, tumor suppression,p53, metastasis

ZhenyiSu
contact affiliation	Columbia University Medical Center
contact email	zs2391@cumc.columbia.edu
lab head
huanli
contact affiliation	COLUMBIA UNIVERSITY MEDICAL CENTER
contact email	hl2977@cumc.columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039886

PRIDE project URI

• PRIDE
  1. PXD039886
     1. Label: PRIDE project
     2. Name: Distinct gain of function mechanism for the hot-spot mutant p53R175H