PXD035795

PXD035795 is an original dataset announced via ProteomeXchange.

Title	Sulfide regulation through persulfidation under non-photorespiratory conditions in Arabidopsis
Description	Hydrogen sulfide is an important signaling molecule in plants that regulates essential biological processes through protein persulfidation. Although numerous persulfidated proteins were found to be involved in photorespiration, little was known about sulfide-mediated regulation of photorespiration. Label-free quantitative proteomic analysis has revealed a high impact on the protein persulfidation level when plants grown under non-photorespiratory conditions are transferred to air, with the 97 % of total identified proteins more persulfidated under suppressed photorespiration. A detailed study of the effect of sulfide on important aspects associated with photorespiratory growth conditions has provided insight into the role of sulfide in protecting plants grown under suppressed photorespiration. In these conditions, sulfide amends the metabolic imbalance of carbon/nitrogen and restores ATP levels to concentrations similar to those in air grown conditions. Sulfide also plays an essential role in balancing the significant high level of ROS measured in plants under non-photorespiratory conditions to reach a similar cellular redox state in air-grown plants, through the regulation of antioxidative defenses. Furthermore, sulfide regulates another important characteristic resulting from suppression of photorespiration, such as the stomata closure, to decrease the high guard cell ROS levels and inducing the stomata aperture. In this way, sulfide signals the movement of the CO2-dependent stomata to achieve a scenario similar to that of plants growing under normal air conditions, in the opposite direction of the already established ABA-dependent movement of the stomata. Therefore, our findings suggest that the high persulfidation level under suppressed photorespiration reveals an essential role of sulfide signaling under these conditions, and with respect to stomatal movement, the outcome of this signaling is dependent on the growth/stress condition under study.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:36.078.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035795
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thibaut Vignane
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-04 07:47:07	ID requested
1	2023-02-26 11:12:17	announced
⏵ 2	2023-11-14 08:57:36	announced	2023-11-14: Updated project metadata.

10.6019/PXD035795;

submitter keyword: Arabidopsis,hydrogen sulfide, High CO2, persulfidation, ROS, photorespiration, stomatal movement

Dr. Milos Filipovic
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS - eV, Sulfaging group
contact email	milos.filipovic@isas.de
lab head
Thibaut Vignane
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS - eV
contact email	thibaut.vignane@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035795

PRIDE project URI

• PRIDE
  1. PXD035795
     1. Label: PRIDE project
     2. Name: Sulfide regulation through persulfidation under non-photorespiratory conditions in Arabidopsis