PXD028595

PXD028595 is an original dataset announced via ProteomeXchange.

Title	Discovery of kinetochore and spindle assembly checkpoint proteins in apicomplexan parasites
Description	Kinetochores are multiprotein assemblies that bind chromosomes to the spindle during mitosis. Despite conserved roles, repertoires of kinetochore proteins vary greatly across eukaryotes. In particular, most known components are not clearly detected in a group of parasites known as the Apicomplexa. Furthermore, flexibility in scale of amplification and an apparent incapability to delay cell cycle progression in response to spindle integrity has coined an idea these parasites divide in the absence of spindle checkpoints. In this study, we reunite divergent apicomplexan kinetochore components to a common eukaryotic set and additionally identify 9 kinetochore proteins that share little homology to known proteins. AKiTs are essential for chromosome segregation and show modes of division parallel to eukaryotic metaphase to anaphase transition and spindle assembly checkpoint signaling. These findings suggest conserved spindle assembly checkpoint signaling maintains fidelity during chromosome segregation in apicomplexan parasites.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:26:27.176.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028595
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	alexandre Hainard
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 23:06:28	ID requested
1	2023-03-10 17:14:34	announced
⏵ 2	2023-11-14 08:26:28	announced	2023-11-14: Updated project metadata.

Brusini L, Dos Santos Pacheco N, Tromer EC, Soldati-Favre D, Brochet M, Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation. J Cell Biol, 221(9):(2022) [pubmed]

10.6019/PXD028595;

submitter keyword: spindle assembly, LC-MSMS,apicomplexan parasite, immunoprecipitation

Mathieu Brochet
contact affiliation	Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, CH-1211 Geneva, Switzerland.
contact email	mathieu.brochet@unige.ch
lab head
alexandre Hainard
contact affiliation	University of Geneva
contact email	alexandre.hainard@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028595
     1. Label: PRIDE project
     2. Name: Discovery of kinetochore and spindle assembly checkpoint proteins in apicomplexan parasites