The evaluation of myocardium characterization is an important role in cardiac MRI. The dark-blood (DB) sequence is used for non-enhanced T2-weighted myocardial images. The purpose of this study was to evaluate and optimize the DB T2WI sequence in myocardium images. We changed the parameters TE, echo train length (ETL), inversion time (TI), and fat suppression method, respectively. Data acquisition with end-diastolic phase was effective for avoiding the blur that was caused by cardiac motion. Consequently, the optimal setting of ETL and TI was important for myocardial images. STIR was suitable for fat suppression in unstable susceptibility. Setting the parameters synchronized with the cardiac cycle provided optimal myocardial images in DB T2WI.