In recent years, considerable interest has been stimulated in the effect of various diseases upon the metabolic pathway by which L-Tryptophan is converrted to nicotinic acid derivatives. Since the present method of determination of these metabolites is complicated and time consuming we have developed a method which is simple and accurate. Using Gas Chromatography techniques we have separated eleven of the Tryptophan metabolites (Nicotinic acid, O-aminophenol, Picolinic acid, Anthranilic acid, 3-hydroxy-anthranilic acid, Quinolinic acid, Quinaldic acid, Kynurenic aicd, DL-kynurenine, Xanthurenic acid, and 3-hydroxy-DL-kynurenine.) utilizing N, O-bis-trimethylsilylacetamide (BSA) in Pyridine to prepare the trimethylsilyl derivatives. A glass column 2m long 4mm, i. d., was utilized, which contained 2% OV-17 Gas chrom Q (80-100mesh) with a nitrogen carrier gas flow of 30ml/min. and FID. The column temperature was changed at a rate of 3°C/min, starting from the initial temperature of 130°C.