It has been found that low energy laser exposure can affect (biostimulate) various processes in cells and tissues. The effects of direct He-Ne laser on the histopathology of a primary culture of satellite cells isolated from rat skeletal muscle was investigated using electron microscopy. The cultured cells were directly exposed to laser light for 3s, and were prepared for electron microscopy 4 days post-irradiation. Non-laser-irradiated (NLI) cells served as a control. The treated cells exhibited well-developed rough endoplasmic reticulum, mitochondria and other cell components, similar to the NLI satellite cells. Previously, we showed that the same dose of laser therapy enhances satellite cell proliferation twofold. Taken together, we suggest that direct He-Ne laser treatment at the power density that can enhance satellite cell proliferation in culture does not cause histopathological changes in the cells, as manifested by their ultrastructure.