African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12482

Full Length Research Paper

Isolation and molecular characteristics of extended spectrum beta-lactamase-producing uropathogenic Escherichia coli isolated from hospital attendees in Ebonyi State, Abakaliki

Ugbo E. N.
  • Ugbo E. N.
  • Department of Applied Microbiology, Faculty of Sciences, Ebonyi State University, P. M. B. 053, Abakaliki, Ebonyi State, Nigeria.
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Anyamene C. O.
  • Anyamene C. O.
  • Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
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Moses I. B.
  • Moses I. B.
  • Department of Applied Microbiology, Faculty of Sciences, Ebonyi State University, P. M. B. 053, Abakaliki, Ebonyi State, Nigeria.
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Ariom T. O.
  • Ariom T. O.
  • Institute of Food Security, Environmental Resources and Agricultural Research, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
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Agumah N. B.
  • Agumah N. B.
  • Department of Applied Microbiology, Faculty of Sciences, Ebonyi State University, P. M. B. 053, Abakaliki, Ebonyi State, Nigeria.
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Chukwunwejim C. R.
  • Chukwunwejim C. R.
  • Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.
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Egbule C. U.
  • Egbule C. U.
  • Department of Science Laboratory Technology, Akanu-Ibiam Federal Polytechnology, Unwana, P.M.B. 1007, Ebonyi State, Nigeria.
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Emioye A. A.
  • Emioye A. A.
  • Department of Biology/Microbiology/Biotechnology, Federal University Ndufu-Alike, Ikwo Ebonyi State, Nigeria.
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Okata-Nwali O. D.
  • Okata-Nwali O. D.
  • Department of Biology/Microbiology/Biotechnology, Federal University Ndufu-Alike, Ikwo Ebonyi State, Nigeria.
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Aneke C. J.
  • Aneke C. J.
  • Department of Applied Biochemistry, Faculty of Natural and Natural Sciences, Enugu State University of Science and Technology, Agbani, Nigeria.
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Ugadu I. O.
  • Ugadu I. O.
  • National Veterinary Research Institute, P. M. B. 01, Vom, Plateau State, Nigeria.
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Osu B. O.
  • Osu B. O.
  • Department of Applied Microbiology, Faculty of Sciences, Ebonyi State University, P. M. B. 053, Abakaliki, Ebonyi State, Nigeria.
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  •  Received: 24 September 2020
  •  Accepted: 25 November 2020
  •  Published: 30 November 2020

Abstract

This present study was designed to determine the occurrence frequency of blaCTX-M and blaTEM genes in extended-spectrum beta-lactamase (ESBL)-producing uropathogenic Escherichia coli, and their antibiotic resistance patterns among hospital attendees in Abakaliki, Nigeria. Out of the 73 uropathogenic E.coli isolates analyzed, 52 were identified to be ESBL producers using double disk synergy technique. The frequency of beta-lactamse (bla) genes (CTX-M and TEM) among E. coli was molecularly determined by polymerase chain reaction (PCR). Out of 52 (71.2%) ESBL-producing uropathogenic E. coli, 17 (32.7%) were positive for blaTEM, 35 (67.3%) were positive for blaCTX-M while 8 (15.3%) harboured both blaTEM and blaCTX-M genes. CTX-M gene was the most prevalent gene. Isolates evaluated in our study were resistant to cefotaxime (83.6%), ceftazidime (79.5%), amoxycillin (72.6%), cefpodoxime (68.5%), aztreonam (61.6%), ceftriaxone (57.5%), and cefepime (37%). This study demonstrated the occurrence frequency of CTX-M and TEM genes in uropathogenic E. coli strains in Abakaliki. Even though molecular techniques are more reliable in the detection of ESBL production, routine clinical screening for ESBL-producing uropathogens using phenotypic method should be introduced and encouraged in clinical settings as they are less expensive. This will go a long way in checkmating drug resistance.

 

Key words: Escherichia coli, uropathogens, extended-spectrum beta-lactamase (ESBL), resistance genes, antibiotics.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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