Abstract

Sugarcane (Saccharum officinarum L) var. 668 was propagated through tissue culture technique: callogenesis at MS media supplemented with different concentrations of growth regulators. Explants were disinfected with 70% ethanol which was sprayed directly on outer most sheathe which covered the tender stem to be used as source of explants. After 15 days, those explants which survived turned into green leaves and were used for the aforementioned study. Different concentrations of growth regulators were applied. For callogensis response from the cultivated explant, combination of Murashige and Skoog (MS) + 2,4-dichlorophenoxy acetic acid (2,4.D) + benzylaminopurine (BAP) with different concentration was applied and were found best for the establishment of callus from shoot culture.

Key words: Saccharum officinarum L, callogenesis, growth regulators, explants, shoot culture.

Abbreviation

Abbreviations: MS, Murashige and Skoog; 2,4-D, 2, 4- dichlorophenoxy acetic acid; BAP, benzylaminopurine; NAA, naphthalene acetic acid.