Characterization and Antioxidative Activities of Rare C50 Carotenoids-Sarcinaxanthin, Sarcinaxanthin Monoglucoside, and Sarcinaxanthin Diglucoside-Obtained from Micrococcus yunnanensis

Ayako Osawa; Yoko Ishii; Nao Sasamura; Marie Morita; Hiroaki Kasai; Takashi Maoka; Kazutoshi Shindo

doi:10.5650/jos.59.653

Biochemistry and Biotechnology

Characterization and Antioxidative Activities of Rare C₅₀ Carotenoids-Sarcinaxanthin, Sarcinaxanthin Monoglucoside, and Sarcinaxanthin Diglucoside-Obtained from Micrococcus yunnanensis

Ayako Osawa, Yoko Ishii, Nao Sasamura, Marie Morita, Hiroaki Kasai, Takashi Maoka, Kazutoshi Shindo

Author information

Keywords: antioxidative activity, sarcinaxanthin, sarcinaxanthin monoglucoside, sarcinaxanthin diglucoside, Micrococcus yunnanensis

JOURNAL FREE ACCESS

2010 Volume 59 Issue 12 Pages 653-659

DOI https://doi.org/10.5650/jos.59.653

Details

Abstract

While screening for antioxidative carotenoids from marine bacteria, we isolated and identified sarcinaxanthin and its glucosylated compounds (sarcinaxanthin monoglucoside and sarcinaxanthin diglucoside) from a moderately halophilic bacterium-Micrococcus yunnanensis strain AOY-1. In the singlet oxygen (¹O₂) quenching model, the IC₅₀ values of the antioxidative activities of these carotenoids were as follows: sarcinaxanthin , 57 μM; sarcinaxanthin monoglucoside, 54 μM; and sarcinaxanthin diglucoside, 74 μM. In addition, the complete proton nuclear magnetic resonance (¹H NMR) assignments of sarcinaxanthin monoglucoside pentaacetate and sarcinaxanthin diglucoside octaacetate, and fast atom bombardment mass spectrometry/mass spectrometry (FAB-MS/MS) analyses of sarcinaxanthin and sarcinaxanthin monoglucoside are reported for the first time.

Corresponding author

Register with J-STAGE for free!