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Media Optimization for Depolymerization of Alginate by Pseudomonas aeruginosa AG LSL-11

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Abstract:

An agar degrading bacterium Pseudomonas aeruginosa AG LSL-11 was acclimatized to alginate for the production of alginase. Production parameters such as pH, temperature, influence of simple carbohydrates and nitrogen sources, and effect of NaCl on growth and alginase production were carried out. Maximum growth was observed at pH 9.0 and 35 °C, while alginase was produced optimally at pH 9.0 and 30 °C. The alginase produced by Pseudomonas aeruginosa AG LSL-11 was inducible by alginate, and repressed by other simple sugar when supplemented along with alginate in the medium. The bacterium did not require NaCl for growth and production of alginase. The activity staining of partially purified culture supernatant after native PAGE revealed the presence of a single alginase.

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Periodical:

International Letters of Natural Sciences (Volume 19)

Pages:

30-39

DOI:

https://doi.org/10.56431/p-e5z1l4

Citation:

Cite this paper

Online since:

July 2014

Authors:

Basawaraj A. Koti, S. Manohar, J. Lalitha*

Keywords:

Alginase, Bacterium, Pseudomonas aeruginosa

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Creative Commons CC BY 4.0

* - Corresponding Author

References

[1] Qing-Da, Gong-Liang, Zhang, Hai-Tao W., Process Biochemistry 43(2008) 842-847.

Google Scholar

[2] Hu X. K., Jiang X. L., Hwang H. M., Eur J Phycol 39(1) (2004) 67-71.

Google Scholar

[3] Lakshmikanth M., Manohar S., Patnakar J., Vaishampayan P., Souche Y., Lalitha J., World J Microbiol Biotechnol. 22 (2006) 531- 537.

DOI: 10.1007/s11274-005-9068-2

Google Scholar

[4] Dygert S. L., Li D., Florida and Thoma J. A., Anal Biochem. 13 (1965) 367-374.

Google Scholar

[5] Laemmli U. K., Nature 227 (1970) 680-68.

Google Scholar

[6] Lowry O.H., Rosebrough N.J., Farr AL., Randall R.J., J. Biol. Chem. (1951) 193- 265.

Google Scholar

[7] Gacesa P., Wusteman F. S., Appl. Environ. Microbiol. 56 (1990) 2265–2267.

Google Scholar

[8] Preston L. A., Wong Y., Bender C. L., Schiller N. L., J. Bacteriology 182 (2000) 6429-6439.

Google Scholar

[9] Sugano Y., Nagae H., Inagaki K., Yamamoto T., Terada I., Yamazaki Y., J. Ferment. Bioeng. 6 (1995) 549-54

Google Scholar

[10] Alexeeva Y. V., Ivanova E. P., Zvaygintseva T. N., Mikhailov V.V., Lett Appl Microbiol. 35 (2002) 343-346.

DOI: 10.1046/j.1472-765x.2002.01189.x

Google Scholar

[11] Liyan Li., Xiaolu J., Huashi G., Peng W., Hong G., Appl Biochem Biotechnol 164 (2011) 305-317.

Google Scholar

[12] Thiang Yian, Wong L.A., Preston and Neal L., Annu. Rev. Microbiol. 54 (2000) 289-340.

Google Scholar

[13] Hee-Sook M., Choul G.L., Eun Y.L., Review Biotechnol and Bioproc Eng. 16 (2011) 843-851.

Google Scholar

[14] Basawaraj A. Koti, S. Manohar, J. Lalitha. Biotechnol Bioproc Eng. 18 (2013) 333-341.

Google Scholar

[15] Tang H., Taniguchi H., Chu, Q., Zhou, S. Nagata., Letters in Applied Microbiology 48 (2009) 38-43.

Google Scholar

[16] Lixiang C, Lujing X, Xiaoli X, Hongming T, Yuhuan L., Shining Z., J. Agric. Food Chem. (2007) 55.

Google Scholar

[17] Basawaraj A. Koti, S. Manohar, J. Lalitha, Preparative Biochemistry & Biotechnology 42 (2012) 364-377. ( Received 25 June 2014; accepted 04 July 2014 )

Google Scholar

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