Scintigraphic Assessment of Renal Function Using 99mTc-DTPA in Miniature Pigs with Unilateral Ureteral Obstruction

Se Eun Kim; Kyung Mi Shim; Won Guk Lee; Seok Hwa Choi; Soo Hyun Park; Ho Jae Han; Seong Soo Kang

doi:10.5625/lar.2010.26.1.103

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Kim, Shim, Lee, Choi, Park, Han, and Kang: Scintigraphic Assessment of Renal Function Using 99mTc-DTPA in Miniature Pigs with Unilateral Ureteral Obstruction

Original Article

Lab. Anim. Res. 2010;26(1):103-108.

Published online: 17 March 2010

DOI: https://doi.org/10.5625/lar.2010.26.1.103

Scintigraphic Assessment of Renal Function Using ^99mTc-DTPA in Miniature Pigs with Unilateral Ureteral Obstruction

Se Eun Kim¹, Kyung Mi Shim², Won Guk Lee³, Seok Hwa Choi⁴, Soo Hyun Park¹, Ho Jae Han¹, Seong Soo Kang^1,^∗

¹College of Veterinary Medicine and Biotechnology Research Institute, Chonnam National University, Gwangju, Korea

²Department of Radiology, Nambu University, Gwangju, Korea

³Department of Nuclear Medicine, College of Medicine, Chungbuk National University, Cheongju, Korea

⁴College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea

^∗Corresponding author: Seong Soo Kang, Department of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, 300 Yongbong-Dong, Buk-gu, Gwangju, 500-757, Korea Tel: +82-62-530-2877 Fax: +82-62-530-2809 E-mail: vetkang@chonnam.ac.kr

Received 11 November 2009 Revised 9 March 2010 Accepted 18 March 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was performed to investigate the proper method for evaluating renal function in miniature pigs with unilateral ureteral obstruction. Experimental unilateral renal damage was induced after ligation of unilateral right ureter in 3 miniature pigs. On the 3rd postoperative day, scintigraphic images were obtained after 12 mCi of ^99mTc-diethylentriamene pertaacetate (DTPA) intravenous injection. Renography showed that radiopharmaceutical uptakes in the right kidney were lower than those of left kidney uptakes as early as at 3 days after surgical operation. The static images of ^99mTc-DTPA enabled us to measure the relative renal function in miniature pigs with unilateral ureteral obstruction. In conclusion, renography using ^99mTc-DTPA was the useful diagnostic method to evaluate the renal function in miniature pigs.

Keywords: ^99mTc-diethylentriamene pertaacetate (DTPA), unilateral ureteral obstruction, renogram, miniature pig

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Figure. 1.

Dynamic images in a miniature pig with unilateral ureteral obstruction after intravenous injection of 12 mCi of ^99mTc-DTPA (Left: normal kidney, Right: kidney with unilateral ureteral obstruction).

Figure. 2.

Posterior dynamic images of a miniature pig with unilateral ureteral obstruction following injection of ^99mTc-DTPA. (A) perfusion phase, (B) excretion phase.

Figure. 3.

Schematic drawing of region of interest. (A) perfusion phase, (B) excretion phase (Left: normal kidney, Right: kidney with unilateral ureteral obstruction).

Figure. 4.

^99mTc-DTPA time-activity curves from a miniature pig with unilateral ureteral obstruction (Left: normal kidney, Right: kidney with unilateral ureteral obstruction). (A) perfusion time-activity curve (time: seconds), (B) excretion time-activity curve (time: minutes).

Table 1.

Values derived from time-activity curve of renogram in miniature pigs (Mean±SD)

Parameter	Normal	Unilateral ureteral obstruction
Kidney area (cm²)	65.97±13.80	74.18±22.08
Time of peak (min)	3.14±0.48	31.75±0.460
Excretion half time (T^1/2, min)	12.31±0.250	NA
Uptake percentage	63.70±5.140	36.30±5.140

NA: non-available.

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