Short communication: Identification of cultivated and wild Vaccinium species grown in Portugal

  • Márcia Carvalho University of Trás-os-Montes and Alto Douro (UTAD), Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), 5000-801 Vila Real
  • Manuela Matos University of Trás-os-Montes and Alto Douro (UTAD), Dept. of Genetics and Biotechnology, 5000-801 Vila Real University of Lisboa, Faculty of Sciences, BioISI– Biosystems & Integrative Sciences Institute, Campo Grande, Lisboa
  • Valdemar Carnide University of Trás-os-Montes and Alto Douro (UTAD), Centre for Research and Technology of Agro-Environment and Biological Sciences (CITAB), 5000-801 Vila Real University of Lisboa, Faculty of Sciences, BioISI– Biosystems & Integrative Sciences Institute, Campo Grande, Lisboa
Keywords: blueberry, bilberry, genetic diversity, differentiation, molecular marker

Abstract

Vaccinium crops offer a variety of benefits for human health due their high levels of antioxidants. Genetic diversity between two Vaccinium species (sixteen cultivars of Vaccinium corymbosum and three wild populations of Vaccinium myrtillus) were evaluated using Inter Simple Sequence Repeat (ISSR) markers. In V. corymbosum 74 polymorphic markers corresponding to 83.2% of polymorphism were obtained while in V. myrtillus only four polymorphic markers corresponding to 83.2% and 10.6% of polymorphism were observed. The dendrogram obtained showed a clear division into two distinct groups corresponding to the two analyzed species. V. corymbosum group is divided in different sub-clusters based on cultivars pedigree relationships. Twenty-eight specific bands were detected in total; 6 for V. corymbosum and 22 for V. myrtillus. Results allowed the selection of five primers due it potential to detect specific bands in the two species. These markers could be useful for identifying species and cultivars and consequently help in the management of germplasm collections and in breeding programs.

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Published
2018-12-19
How to Cite
Carvalho, M., Matos, M., & Carnide, V. (2018). Short communication: Identification of cultivated and wild Vaccinium species grown in Portugal. Spanish Journal of Agricultural Research, 16(3), e07SC01. https://doi.org/10.5424/sjar/2018163-12502
Section
Plant breeding, genetics and genetic resources