Published online Sep 30, 2008.
https://doi.org/10.5395/JKACD.2008.33.5.481
The remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel
Abstract
The purpose of this study is to observe and compare the remineralization tendencies of artificially demineralized enamel by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel).
For this study, 36 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 3 days in order to produce dental caries artificially that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffered remineralization solution of three different degrees of saturation (0.25, 0.30, 0.35) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (× 100). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were determined.
In conclusion, in the group with the lowest degree of saturation, remineralization occurred thoroughly from the surface to the subsurface lesion, whereas in the groups with greater degree of saturation showed no significant change in the subsurface lesion, although there was corresponding increase in the remineralization width on the surface zones.
Figure 1
Specimen used in the experiment.
Figure 2
Polarizing microscopic observation of demineralized enamel (Group 1, × 100).
Figure 3
Polarizing microscopic observation of remineralized enamel (Group 1, × 100).
Figure 4
Polarizing microscopic observation of demineralized enamel (Group 2, × 100).
Figure 5
Polarizing microscopic observation of remineralized enamel (Group 2, × 100).
Figure 6
Polarizing microscopic observation of demineralized enamel (Group 3, × 100).
Figure 7
Polarizing microscopic observation of remineralized enamel (Group 3, × 100).
Figure 8
Change rate of demineralized depth (demineralized depth after remineralization / demineralized depth before remineralization) × 100 (%).
Figure 9
Change rate of surface lesion width (surface lesion width after remineralization / surface lesion width before remineralization) × 100 (%).
Figure 10
Comparison of density in enamel area before and after remineralization (Group 1).
Figure 11
Comparison of density in enamel area before and after remineralization (Group 2).
Figure 12
Comparison of density in enamel area before and after remineralization (Group 3).
Figure 13
Change rate of mineral density before and after remineralization ((demineralized area before remineralization - demineralized area after remineralization / demineralized area before remineralization) × 100 + 100 (%))
Table 1
Initial composition of demineralization solution
Table 2
Initial composition of remineralization solution
Table 3
One-way ANOVA on the remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel
Table 4
Quantitative value change (%) and the post-hoc result of enamel during de- and remineralization
References
-
Brudevold F, McCann HG. Enamel solubility tests and their significance in regard to dental caries. Ann N Y Acad Sci 1968;153:20–51.
-
-
Margolis HC, Moreno EC. Kinetics of hydroxyapatite dissolution. J Dent Res. 1990Abstr. #1546.
-
-
Lee CY. Artificial Caries Formation in Acid Buffering Solution. Yonsei Dent J 1992;7:34–41.
-
-
Han WS, Kum KY, Lee CY. The Influence of Fluoride on Remineralization of Artificial Dental Caries. J Korean Acad Conserv Dent 1996;21:161–173.
-
-
Nikiforuk MJ. Environmental hypersensitivity living in a hostile world. Can Nurse 1985;81:42–46.
-
-
Park JW, Hur B, Lee CY. The Effects of the Degree of Saturation of Acidulated Buffer Solutions in Enamel and Dentin Remineralization and AFM Observation of Hydroxyapatite Crystals. J Korean Acad Conserv Dent 2000;25:459–473.
-
-
Kim MK, Kum KY, Lee CY. The Influence of pH on Remineralization of Artificial Dental Caries. J Korean Acad Conserv Dent 1997;22:193–208.
-
-
Kim SC, Lee CY. The Effect of the pH of Remineralized Buffer Solutions on Dentin Remineralizxation. J Korean Acad Conserv Dent 2007;32:151–161.
-
-
ten Cate JM. In vitro studies on the effects of F on de- and remineralization. J Dent Res 1990;69:614–619.
-
-
Arends J, Jongebloed W, Ogaard B, Rolla G. SEM and microradiographic investigation of initial enamel caries. Scand J Dent Res 1987;95:193–201.
-
-
Strang R, MacDonald I, Creanor SL, Stephen KW. Mineral content variations in demineralized enamel (abstract). Caries Res 1986;20:185.
-
-
Nancollas GH, Purdie N. The kinetics of crystal growth. Q Rev 1964;18:1–20.
-
-
Park SH, Lee CY, Lee JS. The Effect of Acid Concentration and pH of Lactate Buffer Solution on the Progress of Artificial Caries Lesion in Human Tooth Enamel. J Korean Acad Conserv Dent 1993;18:277–290.
-
-
Kum KY, Lee CY. The Effect of Four Kinds of Acid and Concentration on the Formation of Artificial Carious Lesion in Human Tooth Enamel. J Korean Acad Conserv Dent 1996;21:470–488.
-