Aphids curtail the impact of feeding damage by limiting oligogalacturonide release and suppressing cell wall associated immunity
Creators
- 1. John Innes Centre
- 2. Sapienza University
Description
Aphids are insect pests that use piercing-sucking mouthparts to navigate into leaf tissues and feed from phloem sap. During feeding and probing, aphids penetrate the rigid plant cell walls and deliver effector proteins mixed with saliva into the host plant cells. Cell wall damage usually leads to the release of damage-associated molecular patterns (DAMPs), including oligogalacturonides (OGs), which promote pattern recognition receptor (PRR)-triggered immunity (PTI) against pathogens. Whether OG-induced plant defence responses play a role in aphid-plant interactions is not fully understood. Here we show that OGs are released in aphid-exposed leaves but less so than in non-treated plants, indicating that these insects may suppress the accumulation of OGs during feeding. Prior exposure of plants to OGs induces plant defense responses that reduce the ability of M. persicae to colonize plants. This plant defense is suppressed by the aphid effector Mp10 (also known as chemosensory protein 4 or CSP4) that is delivered by aphids into the cytoplasm of plant cells. Mp10 suppresses PTI responses to OGs and a variety of other elicitors. In understanding it’s mode of action we have found a possible role for Mp10 in the regulation of PTI via the trafficking of cell surface PRRs. Therefore, aphids actively manage the impact of feeding damage by limiting OG release and suppressing cell wall associated immunity.
Notes
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POSTER_MPMI2023_MG7pdf.pdf
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Additional details
Funding
- Recognition BBS/E/J/000PR9795
- UK Research and Innovation
- Resistance: DNA methylation and the evolution of pesticide-resistance genes in aphids BB/R009481/1
- UK Research and Innovation
- Susceptibility BBS/E/J/000PR9797
- UK Research and Innovation
- Response BBS/E/J/000PR9796
- UK Research and Innovation
- All Aphid Effectors on DEK BB/V008544/1
- UK Research and Innovation
References
- Bass et al. (2014). The evolution of insecticide resistance in the peach potato aphid, Myzus persicae. Biochem Mol Biol 51: 41-51.
- Bos et al. (2010). A functional genomics approach identifies candidate effectors from the aphid species Myzus persicae (green peach aphid). PLoS Genetics 6(11): e1001216.
- Mugford et al. (2016). An immuno-suppressive aphid saliva protein is delivered into the cytosol of plant mesophyll cells during feeding. Mol Plant Microbe Interact 29(11): 854-861.
- Silva-Sanzana et al. (2019). Pectin methylesterases modulate plant homogalacturonan status in defenses against the aphid Myzus persicae. Plant Cell 31(8): 1913-1929.
- Deshoux et al. (2022). Role of acrostyle cuticular proteins in the retention of an aphid salivary effector. Int J Mol Sci 23(23):15337.
- Agrios (2005). Plant diseases caused by viruses. Plant Pathology (Fifth Edition), 723-824. DOI: 10.1016/B978-0-08-047378-9.50020-8.
- De Mori (2020). Molecular tools to investigate Sharka disease in Prunus species. Applied Plant Biotechnology for Improving Resistance to Biotic Stress, 203-223. DOI: 10.1016/B978-0-12-816030-5.00010-0.
- Zitter and Murphy (2009). Cucumber mosaic. The Plant Health Instructor. DOI: 10.1094/PHI-I-2009-0518-01.