J Korean Acad Periodontol. 1998 Dec;28(4):837-854. Korean.
Published online Dec 31, 1998.
Copyright © 1998 Korean Academy of Periodontology
Original Article

Effects of differentiation/activation factors on the endotoxin-induced expression of MHC class II molecule and integrins, and production of cytokine by monocyte

Byong-Son Choi, Ho Lee, Kwi-Ok Oh and Hyung-Seop Kim
    • Department of Periodontology and Research Institute of Oral Bio-Science, College of Dentistry,Chonbuk National University, Korea.

Abstract

1,25-Dihydroxyvitamin D3(1,25(OH)2D3) and interferon-gamma(IFN-γ) are capable of regulating cells in immune system and are representative differentiation inducer/activator of monocyte/macrophage lineage cells. They may affect the proinflammatory and immune effector functions of monocyte/macrophage activated with immunostimulants such as bacterial endotoxin. The expression of HLA-D, costimulatory adhesion molecules such as ICAM-1(CD54) and Mac-1(CD11b), and secretion of IL-1β were used to evaluate the influence of 1,25(OH)2D3 and IFN-γ on human promyelocytic cell line THP-1 cells. IFN-γ markedly induces the expression of ICAM-1 and HLA-D, and 1,25(OH)2D3 induces the expression of Mac-1 to a lesser extent. LPS alone markedly induces the expression of ICAM-1 molecule without any increase of HLA-D expression. LPS-induced ICAM-1 expression is synergistically increased by IFN-γ priming, but fail to increase Mac-1 and HLA-D expression. IFN-γ, 1,25(OH)2D3, and LPS separately do not induce the secretion of IL-1β by THP-1, however, IL-1β secretion is synergistically increased when THP-1 is exposed to the combination of IFN-γ and LPS. LPS-induced IL-1βproduction by IFN-γ-primed cell is much higher when THP-1 have been previously exposed to 1,25(OH)2D3. In conclusion, these results show that expression of MHC class II and costimulatory adhesion molecules, and production of IL-1β by cells of monocyte/macrophage lineage is highly regulated and monocyte /macrophage differentiation is required for optimal proinflammatory and immune reaction in response to exposure of bacterial endotoxin.

Keywords
Monocyte; MHC Class II; Integrin; Cytokine


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