2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023 (563-566)
АУТОР(И) / AUTHOR(S): Marina Uđilanović, Andrija Ćirić
Е-АДРЕСА / E-MAIL: marinainka95@gmail.com
DOI: 10.46793/ICCBI23.563U
САЖЕТАК / ABSTRACT:
The requirements for method validation are taken from the method ISO 11277:2020 Soil quality – determination of particle size in soil – by sieving and sedimentation method, Annex C table C.1 [1]. The validation was performed on soil samples and on soil reference material (old PT scheme). Validation includes checking the volume of the pipette i.e., calibration of the pipette, correction for the contribution of the dispersing agent, repeatability (RSDr), reproducibility (RSDR), recovery (R), checking bias from the used lab equipment and the bias from purity of use chemicals. As a final part of the validation, the combined measurement uncertainty (Uc) and expanded measurement uncertainty (U) with a confidence level of 95% were calculated. The pipette calibration error is 2.40%. The obtained results are RSRr = 1.38%, RSDR = 13.21%, Recovery = 100.66%, laboratory equipment bias is 2.66%, bias from used chemicals is 0.17%, combined uncertainty is 5.08% and expanded measurement uncertainty is 10.17% by gravimetric determination of clay content, using the pipette method.
КЉУЧНЕ РЕЧИ / KEYWORDS:
3D-QSAR, O-isopropylidene, styryl-lactone, ADME
ЛИТЕРАТУРА / REFERENCES:
- ISO 11277 (2020). Soil quality — Determination of particle size distribution in mineral soil material — Method by sieving and sedimentation.
- AOAC (2016). Association of Official Agricultural Chemists, Appendix F: Guidelines for Standard Method Performance Requirements.
- Eurachem (2019). Measurement uncertainty arising from sampling: A guide to methods and approaches, second edition.
- Association of Chemical Engineers of Serbia (2021). Seminar SHI, Validation of methods in laboratory practice (in Serbian: Savez hemijskih inženjera Srbije, Seminar SHI (2021), Validacija metoda u laboratorijskoj praksi).
- Association of Chemical Engineers of Serbia (2021). Seminar SHI, Assessment of measurement uncertainty of routine analytical methods (in Serbian: Savez hemijskih inženjera Srbije, Seminar SHI (2021), Procena merne nesigurnosti rutinskih analitičkih metoda).
- International standard ISO/IEC 17025 (2017). General requirements for the competence of testing and calibration laboratories.