13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

- Oral Presentation 144

TITLE: The influence of an optical fiber for Endodontic Photodynamic Therapy.

AUTHORS: Garcez AS1, Nunez SC2, Rodriguez HM3, Sabino CP4, Daghastanli N3, Ribeiro MS4.

1 Faculdades São Leopoldo Mandic – São Paulo – Brazil.2 Instituto de Pesquisas em Saude – INPES/CETAO – São Paulo – Brazil3 Universidade Federal do ABC – Santo André – Brazil4 ,Centro de Laser e Aplicações – IPEN/CNEN – São Paulo - Brazil

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S144.

* doi:10.4317/medoral.17643643

http://dx.doi.org/10.4317/medoral.1764364

Abstract

This study analyzed the use of an optical fiber/diffusor to perform antimicrobial photodynamic therapy (aPDT) in endodontics. Fifty freshly extracted human single-rooted teeth were used. Conventional endodontic treatment was performed using a sequence of Pro-tapper system sequence (Dentsply Maillefer Instruments), the canals were contaminated with E. faecalis 3 days’ biofilm. The samples were divided into 5 groups (n=10) and 20 of these teeth had their crown removed. All the teeth and roots received methylene blue for 1 minute and were irradiated with a diode laser (660nm) for 3 minutes as follow: Group 1 – Roots irradiated with a laser tip area of 0,04cm², Group 2 – Roots irradiated with a laser tip area of 0,028cm², Group 3 - Ten teeth were irradiate with the laser the 0.04cm² tip. The forth group (G4) followed the same methodology as group 3 but the irradiation was performed with the smaller tip (0.028 cm²) and in G5 ten teeth were irradiated using a 200-µm diameter fiber/diffusor-coupled to diode laser. Microbiological samples were taken after accessing the canal, after endodontic therapy and after PDT. Groups 1 and 2 showed reduction of 2 logs (99%), Groups 3 and 4 of 1 log (85% and 97%, respectively) and group 5 reduced 4 logs (99,99%). Results suggest that the use of PDT associated to endodontic treatment in root canals infected with E.faecalis with the optical fiber/diffusor showed better results is better than the laser tip used directed at the root canal access.