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<i>In vitro</i> anti-tumor activity in SGC-7901 human gastric cancer cells treated with dandelion extract
Abstract
Purpose: To investigate the mechanisms of cytotoxicity of a dandelion extract against human gastric cancer cell line SGC-7901 cells.
Methods: The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, flow cytometry, and transwell assays were used to investigate the effects of a dandelion extract on cell proliferation, levels of apoptosis, and cell migration, respectively. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assessed the effects of a dandelion extract on the expression levels of genes regulating proliferation and apoptosis.
Results: Dandelion extract exerted strong cytotoxic effects on the cancer cells. After exposure, apoptotic cells increased and cell migration was reduced. RT-qPCR assay revealed that dandelion extract significantly increased anti-proliferative and pro-apoptotic gene expression, including phosphate and tensin homology deleted on chromosome ten (Pten) and Bcl-2 Associated X protein (Bax) mRNA in the gastric cancer cells. The results also indicate that there was decreased pro-proliferative and antiapoptotic gene expression (i.e., extracellular signal-regulated kinase (Erk), Survivin, and B-cell lymphoma 2 (Bcl2) mRNA).
Conclusion: The results suggest that dandelion extract is a potent gastric cancer cell proliferation, survival, and migration inhibitor with potential pharmaceutical applications for the prevention of gastric cancer.
Keywords: dandelion extract, gastric cancer, cytotoxic effect, migration inhibition
