Biwako-seigan fault zone is one of the most active faults in Japan, located around western shore of Lake Biwa. According to The Headquarters for Earthquake Research Promotion (2004), its probability of earthquake within 30 years is from 0.09% to 9%. To simulate strong motion, caused by the rupture of Biwako-seigan fault zone, we have constructed 3D subsurface structural model around Lake Biwa. The 3D subsurface structural model is constructed using the data of geophysical and geological surveys in and around Lake Biwa. We have modified the model by comparing the observed H/V spectral ratio between earthquakes and theoretical one. Using the modified 3D subsurface structural model, we simulated ground motions for moderate earthquakes. As the results, simulated waveforms of ground velocity agree well with observed ones. By the geological method and adjusting theoretical H/V spectral ratio to observed one, it is possible to re-construct 3D subsurface structural model which is applicable to strong motion simulation, even though there are few data of geophysical surveys.