Isolation and Biomolecular Analysis of Canine Parvovirus 2a from Korean Dogs

Lee-Sang Hyeon; Dong-Kun Yang; Eun-Ju Kim; Yu-Ri Park; Hye Jeong Lee; Bang-Hun Hyun

doi:10.4167/jbv.2023.53.1.043

All Issue

2023 Vol.53, Issue 1 Preview Page Next Page

Original Article

Isolation and Biomolecular Analysis of Canine Parvovirus 2a from Korean Dogs

30 June 2023. pp. 43-50

PDF XML

Abstract

Canine parvovirus (CPV) is highly contagious and causes acute hemorrhagic gastroenteritis in puppies. Four variants of CPV-2 are widely distributed worldwide: CPV-2, CPV-2a, CPV-2b, and CPV-2c. However, there have been few molecular characterization studies of CPV-2a circulating in dog populations in South Korea. Here, we isolated and characterized two CPVs: the CPV2101 and CPV2102 isolates. They were confirmed to be CPVs based on their cytopathic effects, indirect fluorescence assays, electron microscopy, real-time PCR, and sequence analysis. Both isolates showed hemagglutination activity with pig erythrocytes and had viral titers ranging from 10^6.5 to 10^5.5 FAID₅₀/mL in A72 cells. The nucleotide and amino acid sequences of VP2 of the isolates were compared with reported CPV strains. The sequence alignment showed that they had 100% nucleotide sequence similarity with each other and grouped with CPV-2a. They had mutations at positions 267, 322, 324, and 440 in the VP2 gene compared to the original CPV-2a strains. A phylogenetic analysis of the VP2 gene of the isolates revealed that they were closely related to the K01708-01 strain reported from South Korea. The two isolates will serve as useful antigens for developing new CPV vaccines.

Keywords

Canine parvovirus

Dogs

VP2

Phylogenetic analysis

References

Nandi S, Kumar M. Canine parvovirus: current perspective. Indian J Virol 2010;21:31-44. 10.1007/s13337-010-0007-y23637476PMC3550768

Martella V, Decaro N, Buonavoglia C. Evolution of CPV-2 and implicance for antigenic/genetic characterization. Virus genes 2006;33:11-3. 10.1007/s11262-005-0034-816791413

Decaro N, Desario C, Addie DD, Martella V, Vieira MJ, Elia G, et al. The study Molecular epidemiology of canine parvovirus, Europe. Emerg Infect Dis 2007;13:1222-4. 10.3201/eid1308.07050517953097PMC2828098

Allison AB, Kohler DJ, Ortega A, Hoover EA, Grove DM, Holmes EC, et al. Host-specific parvovirus evolution in nature is recapitulated by in vitro adaptation to different carnivore species. PLoS pathog 2014;10:e1004475. 10.1371/journal.ppat.100447525375184PMC4223063

Parrish CR, Aquadro CF, Strassheim ML, Evermann JF, Sgro JY, Mohammed HO. Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol 1991;65:6544-52. 10.1128/jvi.65.12.6544-6552.19911942246PMC250707

Appel MJ, Scott FW, Carmichael LE. Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet Rec 1979;105:156-9. 10.1136/vr.105.8.156516347

Tsao J, Chapman MS, Agbandje M, Keller W, Smith K, Wu H, et al. The three-dimensional structure of canine parvovirus and its functional implications. Science 1991;251:1456-64. 10.1126/science.20064202006420

Truyen U. Evolution of canine parvovirus-a need for new vaccines? Vet Microbiol 2006;117:9-13. 10.1016/j.vetmic.2006.04.00316765539

Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, et al. Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 2001;82:3021-5. 10.1099/0022-1317-82-12-302111714979

Decaro N, Buonavoglia C. Canine parvovirus-a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 2012;155:1-12. 10.1016/j.vetmic.2011.09.00721962408PMC7173204

Pérez R, Bianchi P, Calleros L, Francia L, Hernández M, Maya L, et al. Recent spreading of a divergent canine parvovirus type 2a (CPV-2a) strain in a CPV-2c homogenous population. Vet Microbiol 2012;155:214-9. 10.1016/j.vetmic.2011.09.01722014372

Zhou P, Zeng W, Zhang X, Li S. The genetic evolution of canine parvovirus-A new perspective. PLoS One 2017;12:e0175035. 10.1371/journal.pone.017503528362831PMC5376324

Decaro N, Desario C, Elia G, Campolo M, Lorusso A, Mari V, et al. Occurrence of severe gastroenteritis in pups after canine parvovirus vaccine administration: a clinical and laboratory diagnostic dilemma. Vaccine 2007;25:1161-6. 10.1016/j.vaccine.2006.10.02017092616PMC7130793

Drummond AJ, Rambaut A. BEAST: Bayesian evolutionary analysis by sampling trees. BMC evol Biol 2007;7:214. 10.1186/1471-2148-7-21417996036PMC2247476

Shackelton LA, Parrish CR, Truyen U, Holmes EC. High rate of viral evolution associated with the emergence of carnivore parvovirus. Proc Natl Acad Sci U S A 2005;102:379-84. 10.1073/pnas.040676510215626758PMC544290

Geng Y, Guo D, Li C, Wang E, Wei S, Wang Z, et al. Co-circulation of the rare CPV-2c with unique Gln370Arg substitution, new CPV-2b with unique Thr440Ala substitution, and new CPV-2a with high prevalence and variation in Heilongjiang Province, Northeast China. PloS one 2015;10:e0137288. 10.1371/journal.pone.013728826348721PMC4562665

Maya L, Calleros L, Francia L, Hernández M, Iraola G, Panzera Y, et al. Phylodynamics analysis of canine parvovirus in Uruguay: evidence of two successive invasions by different variants. Arch Virol 2013;158:1133-41. 10.1007/s00705-012-1591-523297117

Nakamura M, Tohya Y, Miyazawa T, Mochizuki M, Phung HT, Nguyen NH, et al. A novel antigenic variant of canine parvovirus from a Vietnamese dog. Arch Virol 2004;149:2261-9. 10.1007/s00705-004-0367-y15503211

Battilani M, Ciulli S, Tisato E, Prosperi S. Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy. Virus Res 2002;83:149-57. 10.1016/S0168-1702(01)00431-211864747

Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 53
No :1
Pages :43-50
Received Date : 2023-01-10
Revised Date : 2023-03-27
Accepted Date : 2023-04-04
DOI :https://doi.org/10.4167/jbv.2023.53.1.043

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

All Issue