Determination of Serum Pre-β-Lipoprotein in Normal Individual by Electrophoresis on Cellulose Acetate Membrane

Experimental, epidemiological and clinical studies indicate and important relationship between abnormalities in serum lipoproteins and atherosclerosis. With the introduction in 1965 by Fredrickson and Lees of a system for phenotyping hyperlipoproteinemias, quantitation of pre-and β-lipoproteins gained much significance, and hence, the development of simple method for studying serum lipids and lipoproteins is needed.

In the past, several methods have been proposed for the estimation of serum lipoproteins by electrophoresis.

Authors observed serum total lipoprotein, beta-lipoprotein, pre-beta-lipoprotein and alpha-lipoprotein fractions in 80 (male:40, female;40)normal individuals with electrophoesis using cellulose acetate membrane instead of filter paper and concluded as follow:

1) Total lipoprotein and beta-lipoprotein levels had increasing tendency according to aging on both sexes.

2) Serum pre-beta-lipoprotein level also had increasing tendency according to age on both sexes and these features are more conspicious after fifth decade.

3) Electrophoresis using cellulose acetate membrance seem to be convinient method for analysis of serum lipoproteins especially pre-beta-lipoprotein fraction.

4) This method can be likely applied as routine screening test of hyperlipemia.