Open Access, Peer-reviewed
eISSN 2093-758X
    J Korean Acad Nurs. 2013 Jun;43(3):361-369. Korean.
    Published online Jun 28, 2013.
    https://doi.org/10.4040/jkan.2013.43.3.361
    © 2013 Korean Society of Nursing Science
    Original Article

    Effect of Anorexia and Neuropathic Pain Induced by Cisplatin on Hindlimb Muscles of Rat

    Gee Su Yang,1 and Myoung-Ae Choe2
      • 1School of Nursing, University of Maryland, Baltimore, MD, U.S.A.
      • 2College of Nursing, Seoul National University, Seoul, Korea.
    • Address reprint requests to: Yang, Gee Su. School of Nursing, University of Maryland, 655 West Lombard Street, Room 404, Baltimore, MD 21201, U.S.A. Tel: +1-310-424-0756, Fax: +1-410-706-3769, Email: gsyang@umaryland.edu
    Received September 10, 2012; Accepted March 22, 2013.

    Abstract

    Purpose

    The purpose of this study was to examine the effect of anorexia and neuropathic pain induced by cisplatin on hindlimb muscles of rats.

    Methods

    Adult male Sprague-Dawley rats were divided into two groups, a cisplatin-treated group (n=10) and a control group (n=10). In the cisplatin-treated group, cisplatin at a dose of 2 mg/kg was injected intraperitoneally two times a week up to a cumulative dose of 20 mg/kg over 5 weeks, and in the control group saline (0.9% NaCl) was injected intraperitoneally at the same dose and duration as the cisplatin-treated group. At 34 days all rats were anesthetized, after which the soleus and plantaris muscles were dissected. Withdrawal threshold, body weight, food intake, activity, muscle weight, Type I and II fiber cross-sectional areas and myofibrillar protein content of the dissected muscles were determined.

    Results

    Compared with the control group, the cisplatin-treated group showed significant decreases (p<.05) in withdrawal threshold, activity, food intake, body weight, Type I and II fiber cross-sectional areas, myofibrillar protein content and weight of the soleus and plantaris muscles.

    Conclusion

    Muscular atrophy in hindlimb occurs due to anorexia and neuropathic pain induced by the cisplatin treatment.

    Keywords
    Cisplatin; Neuropathic pain; Anorexia; Muscular atrophy

    Figures

    Figure 1
    Changes of withdrawal thresholds in the control and cisplatin groups.

    Tables

    Table 1
    Experimental Procedures

    Table 2
    Experimental Results in the Control and Cisplatin Groups

    Notes

    This manuscript is based on a part of the first author's master's thesis from Seoul National University.

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    MeSH Terms
    Anorexia*
    Animals
    Body Weight
    Cisplatin/toxicity*
    Eating
    Hindlimb
    Injections, Intraperitoneal
    Male
    Motor Activity
    Muscle Fibers, Skeletal/metabolism
    Muscle Fibers, Skeletal/pathology
    Muscle Proteins/metabolism
    Muscle, Skeletal/drug effects*
    Muscle, Skeletal/physiology
    Neuralgia/chemically induced*
    Neuralgia/pathology
    Rats
    Rats, Sprague-Dawley
    Metrics
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