Metaphase Displacement and Anaphase Lagging during Induced Lymphocyte Culture in Cattle and Buffalo

Chromosomal aberrations are of great concern in dairy animals worldwide as they are usually associated with reduced fertility or infertility. There are multiple reasons which may cause chromosomal aberrations during mitosis and meiosis. To understand the probable cause of aberrations, a simple study was performed during induced lymphocyte culture. The lymphocyte cultures were setup using the blood samples of cattle and buffaloes. Samples were harvested without adding ethedium bromide and colchicine. The Giemsa stained chromosome slides were screened under the microscope (100X) and photographed with attached system.

DOI: http://dx.doi.org/10.4038/cjsbs.v42i1.5900

Ceylon Journal of Science (Bio. Sci.) 42 (1): 63-66, 2013