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HomeKey Engineering MaterialsKey Engineering Materials Vol. 782Enzyme Immobilization Behavior on the Surface of...

Enzyme Immobilization Behavior on the Surface of Hydroxyapatite Capsules under Alkaline Condition

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Abstract:

We prepared hydroxyapatite (HA) capsules encapsulating maghemite particles. In order to evaluate enzyme immobilization behavior of the HA capsules under alkaline condition, we immobilized five kinds of enzymes with different isoelectric point in carbonate/bicarbonate buffer (CBB, pH 10.0). When the enzymes in CBB were moderately charged, immobilization efficiency on the HA capsules showed the highest value. It was suggested that immobilization efficiency was affected according to both pI of enzyme and pH of the surrounding solution and that enzyme immobilized on the HA capsules by not only electrical double layer interactions but also ion interaction and other interactions.

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Bioceramics 30

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Periodical:

Key Engineering Materials (Volume 782)

Pages:

21-26

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.782.21

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Online since:

October 2018

Authors:

Takeshi Yabutsuka*, Masaya Yamamoto, Shigeomi Takai, Takeshi Yao

Keywords:

Alkaline Condition, Capsules, Enzyme Immobilization, Hydroxyapatite, Maghemite

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* - Corresponding Author

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