A QCM-D Study of Reduced Antibody Fragments Immobilized on Planar Gold and Gold Nanoparticle Modified Sensor Surfaces

Asta Makaraviciute; Tautgirdas Ruzgas; Arunas Ramanavicius; Almira Ramanaviciene

doi:10.4028/www.scientific.net/KEM.605.340

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 605A QCM-D Study of Reduced Antibody Fragments...

A QCM-D Study of Reduced Antibody Fragments Immobilized on Planar Gold and Gold Nanoparticle Modified Sensor Surfaces

Abstract:

An immunosensor is an analytical system consisting of specific immune system molecules coupled to a signal transducer. Immunosensor sensitivity depends on the type of immunorecognition ligands used, immobilization influence on their activity and orientation on the surface. Quartz crystal microbalance with dissipation (QCM-D) was employed to investigate the immobilization of antibodies against bovine leukemia virus antigen gp51 (gp51). Disulphide bridges of antibody hinge region were reduced chemically to yield two half antibody fragments (Frag-Ab), each having a single antigen binding site and free sulfhydryl groups that were used for immobilization. Frag-Ab were immobilized on planar gold and gold nanoparticle (AuNP) modified QCM-D sensor surfaces from initial solutions of different concentrations. Higher Frag-Ab surface density values were obtained on AuNP modified surfaces at all tested antibody concentrations. Frag-Ab/gp51 specific interaction was registered and it was determined that the highest sensitivity was exhibited by Frag-Ab immobilized at the lowest surface desities on both types of investigated surfaces. Specific gp51 interaction with Frag-Ab and non-specific binding to bovine serum albumin modified surfaces were compared by employing Δf/ΔD plots, which could serve as fingerprints of different processes.

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Periodical:

Key Engineering Materials (Volume 605)

Pages:

340-343

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.605.340

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Online since:

April 2014

Authors:

Asta Makaraviciute*, Tautgirdas Ruzgas, Arunas Ramanavicius, Almira Ramanaviciene

Keywords:

Antibody, Antibody Fragments, Biosensor, Gold Nanoparticle, Immunosensor, QCM-D

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* - Corresponding Author

References

[1] A. Montrose, S. Cargou, F. Nepveu, R. Manczak, A.-M. Gué, and K. Reybier: Biosens. Bioelectron. 49 (2013), p.305

DOI: 10.1016/j.bios.2013.05.025

Google Scholar

[2] O. Cubuk, M. Altikatoglu, V. Erci, I. Isildak, and N. Tinkilic: Sensor Lett. 11 (2013), p.585

DOI: 10.1166/sl.2013.2735

Google Scholar

[3] O. Laczka, L. Skillman, W. G. Ditcham, B. Hamdorf, D. K. Y. Wong, P. Bergquist, and A. Sunna: J. Microbiol. Methods 95 (2) (2013), p.182

DOI: 10.1016/j.mimet.2013.08.014

Google Scholar

[4] F. Conzuelo, S. Campuzano, M. Gamella, D. G. Pinacho, A. J. Reviejo, M. P. Marco, and J. M. Pingarrón: Biosens. Bioelectron. 50 (2013), p.100

DOI: 10.1016/j.bios.2013.06.019

Google Scholar

[5] Y. Wu, H. Niu, K. Wang, Q. Liu, H. Li, X. Dong, K. Zhao, and Y. Zhu: Sensor Lett. 11 (2013), p.436

Google Scholar

[6] A. Makaraviciute and A. Ramanaviciene: Biosens. Bioelectron. 50 (2013), p.460

Google Scholar

[7] Z. Balevicius, A. Ramanaviciene, I. Baleviciute, A. Makaraviciute, L. Mikoliunaite, and A. Ramanavicius: Sens. Actuat. B-Chem. 160 (2011), p.555

DOI: 10.1016/j.snb.2011.08.029

Google Scholar