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Paper Titles
Physiological, Genomic, and Proteomic Characterization of New “Ferrovum” Strains Obtained from a Pilot Plant for Mine-Water Treatment
p.149
Predicting the Metabolic Potential of the Novel Iron Oxidising Bacterium "Ferrovum" sp. JA12 Using Comparative Genomics
p.153
Analysis of Gene Expression in Response to Copper Stress in Acidithiobacillus ferrooxidans Strain D2, Isolated from a Copper Bioleaching Operation
p.157
Temporal Dynamics of Genes Involved in Metabolic Pathways of C and N of L. ferriphilum, in the Industrial Bioleaching Process of Escondida Mine, Chile
p.162
Analysis of Gene Expression as Marker of Relevant Metabolisms, in Three Acidithiobacillus ferrooxidans Strains, in Different Growth Conditions
p.166
Variance Calculations for Quantitative Real-Time PCR Experiments with Multiple Levels of Replication
p.172
Optimization of the Method for Simultaneous Extraction Bioleaching Bacteria DNA and RNA
p.177
Optimization of the Separation Method for Bioleaching Bacteria DNA and RNA Extracted Simultaneously
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How the RegBA Redox Responding System Controls Iron and Sulfur Oxidation in Acidithiobacillus ferrooxidans
p.186

Analysis of Gene Expression as Marker of Relevant Metabolisms, in Three Acidithiobacillus ferrooxidans Strains, in Different Growth Conditions

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Abstract:

Acidithiobacillus ferrooxidans is a chemiolithoautotrophic Gram-negative bacterium widely spread in ambient temperature bioleaching processes. Several strains of At. ferrooxidans were isolated and studied and, some time later, questions arise about whether it was a species with a wide metabolic variation or a group of closely related species. Advances in molecular biology, phylogeny and genomics have shed some light on At. ferrooxidans strains and allows their grouping according to their relations. However, significant challenges remains to be met, such as understanding how a particular strain faces environmental challenges and how a particular kind of adaptive response affects the growth and activity of the strain. The purpose of this study was to identify differential expression signals between At. ferrooxidans strains −with different abundances and dynamics− present in the bioleaching system at Escondida mine. Culture characterization and DNA macroarrays techniques provided some answers. Analysis of growth curves showed that IESL 32 had the highest anaerobic growth rate, while aerobic growth was similar for all strains. It was shown that though the phylogenetic analysis based on 16S rRNA sequences suggested a close relation between IESL 32 and the type strain ATCC 23270, the growth curves and the expression profile showed that the type strain and strain D2 had the closest similarity. Growth experiments under different conditions, together with the comparative analysis of gene expressions in At. ferrooxidans, could be a springboard for future investigations of strain characterization to broaden our knowledge about adaptation, metabolic strategies, regulation and microbial diversity in industrial processes.

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Periodical:

Advanced Materials Research (Volume 825)

Pages:

166-171

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.825.166

Citation:

Cite this paper

Online since:

October 2013

Authors:

Gonzalo Araya, Mauricio Acosta, Cecilia Demergasso

Keywords:

Anaerobic Growth, At. ferrooxidans, Gene Expression, Macroarray

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