Absorbance Analysis of Escherichia coli (E. coli) Bacteria Suspension in Polydimethylsiloxane (PDMS)-Glass Based Microfluidic

Nurulazirah Md Salih; Uda Hashim; Nafarizal Nayan; Chin Fhong Soon; Mohd Zainizan Sahdan

doi:10.4028/www.scientific.net/AMR.1133.65

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 1133Absorbance Analysis of Escherichia coli (E. coli)...

Absorbance Analysis of Escherichia coli (E. coli) Bacteria Suspension in Polydimethylsiloxane (PDMS)-Glass Based Microfluidic

Abstract:

The emerging of bacteria/cell culturing in biological/biomedical research and industry is in demand for low cost, fast, non-invasive, and reliable alternative/approach for evaluation and measurement. Microfluidic approach is one of the promising alternatives for replacing the expensive commercial cuvvete for bacteria/cell culture and suspension for optical measurement. This study demonstrates the integration of absorbance measurement with microfluidic platform for Escherichia coli (E. coli) bacteria suspension analysis. The E. coli was cultured and prepared for suspension medium which then transferred inside the PDMS-glass based microfluidic. Then, the absorbance measurement is carried out using UV-Visible spectrophotometer. We demonstrate this method by measuring absorption of light transmitted through microfluidic chambers within the visible light range (350nm - 750nm). From the result, it had indicates that the graph pattern and growth behavior of E. coli suspension in microfluidic platform are reliable and comparable to commercial cuvvete reading. This finding

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Periodical:

Advanced Materials Research (Volume 1133)

Pages:

65-69

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1133.65

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Online since:

January 2016

Authors:

Nurulazirah Md Salih*, Uda Hashim, Nafarizal Nayan, Chin Fhong Soon, Mohd Zainizan Sahdan

Keywords:

Absorbance, Escherichia coli, Microfluidic, Spectrophotometer, Suspension

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