Biomass Production and Fatty Acids Long Chain Polyunsaturated Omega 3 (LC-PUFAs) Chilean Strain Using Thraustochytrids

David Silva; Gabriel Cortínez; Benita H. Quilodrán

doi:10.4028/www.scientific.net/AEF.14.23

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HomeAdvanced Engineering ForumAdvanced Engineering Forum Vol. 14Biomass Production and Fatty Acids Long Chain...

Biomass Production and Fatty Acids Long Chain Polyunsaturated Omega 3 (LC-PUFAs) Chilean Strain Using Thraustochytrids

Abstract:

Global utilization of long-chain polyunsaturated fatty acids (LC-PUFAs) has increased steadily in the last decade which has imposed a huge demand for fish oil as it is currently the only source capable of sustaining the enormous commercial requirements this input worldwide. They are currently using various microorganisms to produce single cells oil (SCO) high in LC-PUFAs, within which highlights the thraustochytrids (TH). The TH is a group of marine protists (pseudofungi) with capacity to produce LC-PUFAs such as eicosapentaenoic acid (EPA), docosapentaenoic (DPA) and docosahexaenoic acid (DHA). In this study the chilean VAL-B1 strain was grown in glucose, starch and glycerol being obtained biomass production and LC-PUFAs. Biomass values obtained were 1.02 ± 0.32 g/L, 1.25 ± 0.44 g/L and 1.44 ± 0.27 g/L of glucose, glycerol and starch respectively. The profile of LC-PUFAs showed a percentage of omega-3 fatty acids on glucose of 59.34%, 61.50% in starch and glycerol of 47.86%. The greater this value was to omega-3 DHA when starch was used, with 68.16 mg/g being greater than 45.77 mg/g glycerol and 63.89 mg/g in glucose. Therefore, we conclude that VAL-B1 produces more biomass has greater value of omega-3 LC-PUFAs and greater concentration of DHA when starch is used as a source of substrate for cultures.

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Periodical:

Advanced Engineering Forum (Volume 14)

Pages:

23-28

DOI:

https://doi.org/10.4028/www.scientific.net/AEF.14.23

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Online since:

October 2015

Authors:

David Silva*, Gabriel Cortínez, Benita H. Quilodrán

Keywords:

Docosahexaenoic Acid (DHA), Docosapentaenoic Acid (DPA), Eicosapentaenoic Acid (EPA), Long Chain Polyunsaturated Fatty Acids (LC-PUFAs), Thraustochytrids (TH)

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