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Characteristic of Synthetic Coral Scaffold for Cell Environment

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Abstract:

Introduction. Synthetic coral scaffold is fabricated the mimicking of natural sea coral as a scaffold for bone regeneration [1]. Scaffold is performing functions as a micro environment for cells attachment, growth, proliferates, differentiates until it can form new bone tissue. The proper design is needed to produce the scaffold [2]. The purpose of this study was to investigate the characteristics of synthetic coral scaffold for micro environment of cells by observing cell attachment, hydrophobicity, and scaffold porosity. Experimental. Synthetic coral scaffold consists of bovine gelatin dan CaCO3 by weight, the concentration that be used are 4:6. Sodium citrate is used as dispersant. Thick film like scaffold was prepared for this study [1,3]. Vero cell line was used for observing cell attachment to investigate the biocompatibility the scaffold. The hydrophobicity was observed with distilled water droplets dripped on the scaffold surface, be analyzed in a photograph taken by the camera and then measured the angle. Percentage of porosity was measured using Archimedes law in absolute ethanol. Results and Discussion. Vero cells attached successfully into scaffold. Cell viability percentage is 91,77 % from the absorbance value of the MTT assay. It presented that the scaffold has biocompatibility character. However, the percentage of porosity is 55,85%, so the scaffold has enough porosity for cell attachment. Porosity serves for the diffusion of nutrients, gases and removes the residual metabolism resulting from cell activity that has grown on scaffold. The good porosity value of the scaffold is 50-90%. The higher the porosity value the better the scaffold. Hydrophobicity scaffold appears from the contact angle of 81.4°, the cohesion is greater than the adhesion. This shows the greater synthetic coral scaffold hydrophobicity, which is affected by surface roughness from scaffold porosity. The greater hydrophobicity will also prolong the degradation of the scaffold, thereby enabling cells to proliferate, differentiate and produce bone matrix. Conclusions. Synthetic coral scaffold provides the micro environment for cell, high hydrophobicity allows longer degradation for proliferation and differentiation of bone cells, and porosity that allows cells to be inserted within the scaffold.

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Periodical:

Key Engineering Materials (Volume 829)

Pages:

188-193

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.829.188

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Online since:

December 2019

Authors:

Erlina Sih Maharani*, Meibi Murbi Arlianata, Paramita Anindria Putri, Juwita Tiara Normadina, Andiani Refiana Friyandini, Hammia Zanzabiela

Keywords:

Cell Environment, Hydrophobicity, Porosity, Synthetic Coral Scaffold

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* - Corresponding Author

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