Functionalization of Mesoporous SBA-15 with APTES by Co-Condensation and its Effect on Immobilization of Candida rugosa Lipase

Xiu Yan Li; Yun Qiang Xu; Guo Wei Zhou

doi:10.4028/www.scientific.net/KEM.645-646.1261

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HomeKey Engineering MaterialsKey Engineering Materials Vols. 645-646Functionalization of Mesoporous SBA-15 with APTES...

Functionalization of Mesoporous SBA-15 with APTES by Co-Condensation and its Effect on Immobilization of Candida rugosa Lipase

Abstract:

Functionalized SBA-15 with mesoscopic pore channels was synthesized by co-condensation of tetraethoxysilane and (3-aminopropyl) triethoxysilane (APTES) via hydrothermal process. Small-angle X-ray powder diffraction, scanning electron microscopy and transmission electron microscopy were used to monitor the effect of surface functionalization on the structural and textural features of the SBA-15. The results suggested that the structural ordering of functionalized SBA-15, as well as pore diameters, pore volumes and surface areas, were decreased with increasing the APTES molar ratio. Candida rugosa lipase (CRL) was used as a model enzyme for studying the effect of amino-functionalized on loading amount and enzymatic activity. The effects of pH and temperature on catalytic hydrolysis of tributyrin by immobilized CRL were investigated. The results showed that immobilized CRL had a well adaptability in a wide pH and temperature region, and CRL immobilized on functionalized SBA-15 exhibited much higher enzymatic activity than free CRL, especially, 5 mol% APTES functionalized SBA-15 immobilized CRL displayed the highest activity.

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Periodical:

Key Engineering Materials (Volumes 645-646)

Pages:

1261-1266

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.645-646.1261

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Online since:

May 2015

Authors:

Xiu Yan Li, Yun Qiang Xu, Guo Wei Zhou*

Keywords:

(3-Aminopropyl)Triethoxysilane, Candida rugosa Lipase, Co-Condensation, Immobilization, Mesoporous SBA-15

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* - Corresponding Author

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