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Evaluation of RGD-Grafted Porous PLLA Scaffold for Chondrogenic Differentiation of Stem Cells

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Abstract:

Biodegradable porous poly(L-lactic acid) (PLLA) scaffolds were prepared using gas foaming method. The PLLA scaffolds with a hydrophobic surface were subjected to Ar plasma treatment and in situ acrylic acid (AA) grafting to obtain hydrophilic PLLA scaffold (PLLA-PAA). Cell-adhesive RGD peptide was then immobilized onto the AA-grafted PLLA (PLLA-PAA-RGD). Once rabbit bone marrow-derived mesenchymal stem cells (BM-MSC) were isolated, MSCs were seeded into PLLA control, PLLA-PAA, and PLLA-PAA-RGD scaffold and cultured for up to 4 weeks in chondrogenic medium with the addition of 10 ng/ml transforming growth factor (TGF)-β1. Surface analysis of AA-grafted PLLA identified significant alterations of surface characteristics, including reduced contact angle and different atomic compositions. From WST-1 assay at 4 weeks, cells were found more proliferative in PLLA-PAA than the others. Upon the histological analysis of Safranin O staining, chondrogenic differentiation of MSCs appeared to be progressed more actively in PLLA-PAA. The effect of RGD immobilization on MSC differentiation was barely notable.

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Advanced Biomaterials VII View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Pages:

297-300

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.297

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Cite this paper

Online since:

July 2007

Authors:

Hyun Jung Jung, Kwi Deok Park, Kyoung Jin Cho, Kwang Duk Ahn, Dong June Ahn, Dong Keun Han

Keywords:

AA Grafting, chondrogenic Differentiation, RGD, Scaffold, Stem Cell, Surface Modification, Tissue Engineering

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