Bio-Engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion

Chan Wai Chan; Ling Qin; K.M. Lee; H.Y. Yeung; Yun Yu Hu; K.H.K. Wong; R.C.L. Yip; Jack C.Y. Cheng

doi:10.4028/www.scientific.net/KEM.334-335.1201

Paper Titles

Coercivity Dependence of Inductive Heat Property of Fe₃O₄ Nanoparticles in Alternating Current Magnetic Field
p.1185

Fe₃O₄/Polyaniline Nanoparticles with Core-Shell Structure and their Inductive Heating Property in AC Magnetic Field
p.1189

Preparation and Biological Evaluation of Natural Nano-Hydroxypatite/Chitosan Composite
p.1193

Preparation and Evaluation of Self-Hardening Bone-Rehabilitative Composite with Natural Hydroxyapatite/Chitosan
p.1197

Bio-Engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion
p.1201

Objective Quantification of Porous Structure in Orthopaedic Biomaterial Implants Using Micro Computed Tomography
p.1205

Synthesis and Characterization of PEG-Modified ZnGd_0.1Fe_1.9O₄ Ferrite Nanoparticles for Interstitial Hyperthermia to Tumor
p.1209

Formation of Apatite within Biodegradable Scaffolds by an Accelerated Biomimetic Process in the Shaking Condition and Flow Condition
p.1213

Thermophysical and Mechanical Properties of β-Tricalcium Phosphate Reinforced Polyhydroxybutyrate and Polyhydroxybutyrate-Co-Hydroxyvalerate Composites
p.1217

HomeKey Engineering MaterialsKey Engineering Materials Vols. 334-335Bio-Engineered Mesenchymal Stem Cell-Tricalcium...

Bio-Engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion

Abstract:

Grafting of autologous iliac crest and decortication approach in posterior spinal fusion surgery has been the “gold standard”. However, the limited source of autograft has prompted extensive research into bone substitute and biological enhancement of the fusion mass. In this study, the application of stem cell therapy by tissue engineering method was investigated to enhance posterior spinal fusion with -tricalcium phosphate ceramics in rabbit model. Rabbit bone marrow derived mesenchymal stem cells were aspirated from trochanter region of proximal femur. The mesenchymal stem cells were grown and directed to differentiate into osteogenic cells by osteogenic supplement (ascorbic acid, -glycerophosphate and dexamethasone) in basal medium (10% FBS in DMEM). The osteogenic cells were seeded on tricalcium phosphate ceramics for one day (MSC group, n=6). The cell-ceramics composite was implanted onto autologous L5 and L6 transverse processes with decortication approach in posterior spinal fusion. The cell free ceramics acts as control (Control group, n=6) and iliac crest autograft as positive control (Autograft group). The spinal segments were harvested at week 7 post-operation. Manual palpation was performed with spinal segments to assess any movement of L5-L6 vertebral joint. The stiffness of the joint was considered as solid fusion. The specimens then were fixed by formalin and transferred to 70% ethanol. The BMC and volume of fusion transverse processes of L5 and L6 was measured by peripheral quantitative computed tomography. In manual palpation, 50% solid fusion was found in MSC group, 60% in autograft group but none in control group. Moreover, the BMC of L5 and L6 transverse processes in MSC group was greater than autograft and control group (45%, 40% respectively, p<0.01). The volume of transverse processes in MSC group was greater than autograft by 45% (p<0.01) and control group by 26% (p<0.05). In conclusion, the mesenchymal stem cells derived osteogenic cells augmented spinal fusion and bone mineralization.

You might also be interested in these eBooks

Advances in Composite Materials and Structures

View Preview

Info:

Periodical:

Key Engineering Materials (Volumes 334-335)

Pages:

1201-1204

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.334-335.1201

Citation:

Cite this paper

Online since:

March 2007

Authors:

Chan Wai Chan, Ling Qin, K.M. Lee, H.Y. Yeung, Yun Yu Hu, K.H.K. Wong, R.C.L. Yip, Jack C.Y. Cheng

Keywords:

Ceramic, Mesenchymal Stem Cell (MSC), Posterior Spinal Fusion, Tricalcium Phosphate

Export:

RIS, BibTeX

Price:

Permissions:

Request Permissions

References

[1] H.H. Kaufman and E. Jones: Neurosurgery 24 (1989), p.264.

Google Scholar

[2] J.M. Cotler and A.M. Star, in: Complications of Spinal Fusions, edited by J.M. Cotler and H.B. Cotler, in Spinal Fusion: Science and Technique. Springer-Verlag, (1990) pp.361-387.

DOI: 10.1007/978-1-4612-3272-8_17

Google Scholar

[3] J.C.Y. Cheng, X. Guo, L.P. Law, K.M. Lee, D.H.K. Chow and R. Rosier: Spine 27 (2002), p.467.

Google Scholar

[4] X. Guo, L.P. Law, S.K.M. Lee, H.K. Chow, R. Rosier and J.C.Y. Cheng: J. Orthop. Res. 20 (2002), p.740.

Google Scholar

[5] J.C.Y. Cheng, H.Y. Yeung, K.M. Lee, X. Guo, Y.M. Chiu, P. Chow and Y. Tabata Abstract presented at the 7th World Biomaterial Congress, Sydney, Australia (2004).

Google Scholar

[6] C.W. Chan, H.Y. Yeung, K.M. Lee, X. Guo, Y.M. Chiu, P. Chow, Y. Tabata and J.C.Y. Cheng: Key Eng. Mater. 288-289 (2005), p.491.

Google Scholar

[7] G. Cinotti, A.M. Patti, A. Vulcano, D. Della Rocca, G. Polveroni, G. Giannicola and F. Postacchini: J. Bone Joint Surg. [Br] 86B (2004), p.135.

DOI: 10.1302/0301-620x.86b1.14308

Google Scholar

[8] S.M. van Gaalen, W.J. Dhert, A. van den Muysenberg, F.C. Oner, C. van Blitterswijk, A.J. Verbout and J.D. de Bruijn: Tissue Eng. 10 (2004), p.231.

DOI: 10.1089/107632704322791871

Google Scholar

[9] L.X. Bi , D.J. Simmons and E. Mainous: Calcif Tissue Int. 64 (1999), p.63.

Google Scholar

[10] N. Ogura, M. Kawada, W.J. Chang, Q. Zhang, S.Y. Lee, T. Kondoh and Y. Abiko: J. Oral Sci. 46 (2004) p.207.

Google Scholar

[11] T. Cao, B.C. Heng, C.P. Ye, H. Liu, W.S. Toh, P. Robson, P. Li, Y.H. Hong and L.W. Stanton: Tissue Cell 37 (2005), p.325.

Google Scholar