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Antibacterial Effect of Silver Nanoparticles (AgNPs) Synthesized from Trichoderma Harzianum against Clavibacter Michiganensis
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HomeJournal of Nano ResearchJournal of Nano Research Vol. 58Antibacterial Effect of Silver Nanoparticles...

Antibacterial Effect of Silver Nanoparticles (AgNPs) Synthesized from Trichoderma Harzianum against Clavibacter Michiganensis

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Abstract:

In nanotechnology, fungal mediated green synthesis of silver nanoparticles (AgNPs) has tremendous application in the development of antimicrobial systems but the mechanism behind the synthesis is yet to be understood. This study aims to synthesize the silver nanoparticles via a green chemistry route using mycellial aqueous extract of agriculturally beneficial fungi Trichoderma harzianum. Two different concentrations (1 mM and 2.5 Mm) of aqueous silver salt (AgNO₃) were used and mixed as 1:1 ratio with aqueous extract of T. harzianum at room temperature and the pH of the reaction mixture was monitored until it stabilized. Formation of AgNPs was confirmed by using UV-Vis spectroscopic analysis. For further insight, AgNPs were characterized by using HR-TEM and XRD, which clearly showed appearance of crystalline, monodispersed round-shaped particles of 3-20 nm in size. The synthesised NPs were subjected to antimicrobial assay against gram +ve and gram –ve bacteria using the disk diffusion method. The focused species was Clavibacter michiganensis subsp michiganensis, which is the causitive pathogen of Tomato canker disease and we hypothesised that the synthesised AgNPs might be useful to control this pathogen. Appreciable antibiotic activity was monitored even at a low concentration of 1mM level, while the zone of inhibition was positively increased at 2.5 mM. Our results clearly indicate that the present process is an excellent candidate for industrial scale production of AgNPs, and has the potential to control the bacterial pathogen cmm.

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Journal of Nano Research Vol. 58

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Periodical:

Journal of Nano Research (Volume 58)

Pages:

10-19

DOI:

https://doi.org/10.4028/www.scientific.net/JNanoR.58.10

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Online since:

June 2019

Authors:

Asma Noshad*, Mudassar Iqbal, Laura Folkers, Crispin Hetherington, Aamir Khan, Muhammad Numan, Saleem Ullah

Keywords:

AgNPs (Silver Nanoparticles), Antimicrobial Activity, Clavibacter michiganensis (cmm), Green Synthesis, T. Harzianum (Trichoderma harzianum)

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