A Preliminary Differential Proteomic Analysis of the Periplasmic Fraction in Acidithiobacillus Ferrooxidans Planktonic and Attached Cells Exposed to Chalcopyrite

Ana P. Felício; Eliandre de Oliveira; M.A. Odena; Oswaldo Garcia Jr.; Maria C. Bertolini; Lúcio F.C. Ferraz; Laura M.M. Ottoboni; Maria T.M. Novo

doi:10.4028/www.scientific.net/AMR.71-73.179

Paper Titles

Regulation of the Iron and Sulfur Oxidation Pathways in the Acidophilic Acidithiobacillus Ferrooxidans
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Physiological and Phylogenetic Heterogeneity among Iron-Oxidizing Acidithiobacillus spp., and Characteristics of the Novel Species Acidithiobacillus Ferrivorans
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Chromosomal Arsenic Resistance Genes from Sulfobacillus Thermosulfidooxidans and a Demonstration that the Genetic Diversity of arsB among the Sulfobacilli is Similar to that of their 16S rRNA Genes
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Characterization of Biofilm Formation by the Bioleaching Acidophilic Bacterium Acidithiobacillus Ferrooxidans by a Microarray Transcriptome Analysis
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A Preliminary Differential Proteomic Analysis of the Periplasmic Fraction in Acidithiobacillus Ferrooxidans Planktonic and Attached Cells Exposed to Chalcopyrite
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The Secretome of the Extremophilic Acidithiobacillus Ferrooxidans ATCC 23270
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A 300 kpb Genome Segment, Including a Complete Set of tRNA Genes, is Dispensable for Acidithiobacillus Ferrooxidans
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Small Regulatory RNAs in Acidithiobacillus Ferrooxidans: Case Studies of 6S RNA and Frr
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Prediction of FNR Regulated Genes and Metabolic Pathways Potentially Involved in Anaerobic Growth of Acidithiobacillus Ferrooxidans
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 71-73A Preliminary Differential Proteomic Analysis of...

A Preliminary Differential Proteomic Analysis of the Periplasmic Fraction in Acidithiobacillus Ferrooxidans Planktonic and Attached Cells Exposed to Chalcopyrite

Abstract:

The Acidithiobacillus ferrooxidans periplasmic space is known to have proteins involved in the respiratory chains. There are no reports about the expression of the periplasmic proteins in A. ferrooxidans cells attached to chalcopyrite. In this preliminary work, it was compared the periplasmic protein profiles of A. ferrooxidans planktonic and attached cells after exposure to chalcopyrite for 2 hours. The bacterial response to chalcopyrite was investigated by a proteomic approach (two- dimensional gel electrophoresis and mass spectrometry). Four proteins differentially expressed between planktonic and attached cells after exposure to chalcopyrite were identified. Two of these proteins, repressed in chalcopyrite- attached cells, were both identified as superoxide dismutase, whereas the single strand binding protein (SSB) and the PspA/IM30 protein were induced. These results showed that A. ferrooxidans chalcopyrite- attached and planktonic cells show differential expression of the periplasmic proteins and that a proteomic approach can provide a valuable tool to detect proteins related to the A. ferrooxidans response to attachment to the mineral substrates.

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Periodical:

Advanced Materials Research (Volumes 71-73)

Pages:

179-182

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.71-73.179

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Online since:

May 2009

Authors:

Ana P. Felício, Eliandre de Oliveira, M.A. Odena, Oswaldo Garcia Jr., Maria C. Bertolini, Lúcio F.C. Ferraz, Laura M.M. Ottoboni, Maria T.M. Novo

Keywords:

Acidithiobacillus ferrooxidans, Biofilm, Chalcopyrite, Differential Proteomic Analysis, Periplasmic Protein, Two-Dimensional Gel Electrophoresis

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