Surface Modification of Coronary Stents for Intravascular Gene Delivery

Lan Xia Liu; Xiao Hong Li; Chao Zhang; Xi Gang Leng; Cun Xian Song

doi:10.4028/www.scientific.net/AMR.668.265

Paper Titles

Synthesis of RAFT Molecularly Imprinting Polymer Based on Ionic Liquid
p.246

Synthesis and Properties of Novel Polymer Electronic-Phosphorescent Materials
p.250

Temperature Dependent Photoluminescence from Polysilsesquioxane Xerogels
p.255

Preparation of Two Novel Ionic Electroconductive Polyurethanes Functionalized with the Backbone of Ionic Liquid
p.259

Surface Modification of Coronary Stents for Intravascular Gene Delivery
p.265

The Theoretical Research on Development Direction of Cement Grinding Aids
p.269

Preparation and Characterization of Metronidazole Liposome by Supercritical Reverse Phase Evaporation Method
p.274

Improvement of Porous Medium Permeability by Injecting Overheated Steam
p.279

Dissolution Behavior of Cast WC Particles in NiCrBSi-WC Coatings by Laser Induction Hybrid Cladding
p.283

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 668Surface Modification of Coronary Stents for...

Surface Modification of Coronary Stents for Intravascular Gene Delivery

Abstract:

The present study investigated a novel surface modification on metal coronary stent for antibody immobilization. Methods: 316L stainless steel stents were surface modified with protein coatings. An Anti-DNA antibody was covalently bound to the protein surface using a bi-functional cross-linking agent SPDP. The artwork and binding stability of protein coatings were evaluated by in-vitro eluting. The feasibility and stability of anti-DNA antibody covalently bound to the stent were evaluated by means of 125I labeling. Results: We observed that pre-treating the steel surface using diluted HCL and increasing the ratio of cross-linking agent caused significantly increased binding stability of the protein coatings (p﹤0.001). The amount of chemically coupled antibody on the stents was 8 times higher than that of physically absorbed control stents. The stability of chemically coupled antibody on the stent was significantly better than physically absorbed control. Conclusion: It is concluded that we optimized the technique of protein coating on stainless steel and achieved stable anti-DNA antibody immobilization, therefore enabled efficient and highly localized non-viral gene delivery.