The Hydrophobic Effect on Electrodeposition of Billirubin Oxidase CotA

Article Preview

Abstract:

The hydrophobic effect on electrodeposition of an enzyme CotA was investigated. The CotA was electrodeposited on a gold disc electrode modified with an alkane thiol self-assembled monolayer (SAM). The CotA immobilized hydrophobic SAM gold electrodes exhibited a larger electrochemical activity compared with the hydrophilic SAM and bare electrodes. In particular, large current responses were observed in an ethanethiol or a 1-butanethiol SAM gold electrode. In addition, effect of addition of non-ionic surfactant Triton X on the electrodeposition was checked using the SAM gold electrode. The addition of Triton X had a positive effect on the electrodeposition.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

291-294

Citation:

Online since:

June 2015

Export:

Price:

* - Corresponding Author

[1] S. Sakasegawa, H. Ishikawa, S. Imakura, H. Sakuraba, S. Goda and T. Ohshima: Appl. Environ. Microbiol. 72 (2006), 972-975.

DOI: 10.1128/aem.72.1.972-975.2006

Google Scholar

[2] T. Watanabe, Y. Yamada, J. Motonaka, T. Yabutani, H. Sakuraba and M. Yasuzawa: Int. J. Modern Phy. Conf. Ser. 6 (2012), 150-155.

DOI: 10.1142/s2010194512003091

Google Scholar

[3] T. Beneyton, Y. Beyl, D. A. Guschin, A. D. Griffiths, V. Taly, W. Schuhmann: Electroanalysis 23 (2011), 1781-1789.

DOI: 10.1002/elan.201100054

Google Scholar

[4] S. Tsujimura, H. Ttsumi, J. Ogawa, S. Shimizu, K. Kano, T. Ikeda: J. Electroanal. Chem, 496 (2001), 69-75.

Google Scholar

[5] T. Ikeda: Practical Bioelectrochemistry-Recent Developments in Biosensors and Biofuel Cells (CMC publications, Japan 2007).

Google Scholar

[6] N. Matsumoto, X. Chen, and G. S. Wilson: Anal. Chem. 74 (2002), 362-367.

Google Scholar

[7] X. Chen, N. Matsumoto, Y Hu, and G. S. Wilson: Anal. Chem. 74 (2002), 368-372.

Google Scholar

[8] R. K. Mendes, R. F. Carvalhal, L. T. Kubota: J. Electroanal. Chem, 612 (2008), 164–172.

Google Scholar

[9] J. J. Gooding, D. B. Hibbert: Trends in Anal. Chem. 18 (1999), 525-532.

Google Scholar

[10] H. Shen, J. E. Mark, C. J. Seliskar, H. B. Mark, Jr., W. R. Heineman: J. Solid State Electrochem. 1 (1997), 148-154.

DOI: 10.1007/s100080050039

Google Scholar

[11] M. H. Schoenfisch and J. E. Pemberton: J. Am. Chem. Soc. 120 (1998) 4502-4513.

Google Scholar