Screening of Microalgae for Antioxidant Activities, Carotenoids and Phenolic Contents

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Abstract:

Ten different microalgal species were screened for their antioxidant activity using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, both total phenolic and carotenoid contents were determined. The ethanolic extract of Oscillatoria sp. showed the highest antioxidant activity (69.1 %), while the green Chlorella sp. showed higher phenolic content (39.1 mg GAE g-1 dry wt.) and Scenedesmus obliquus recorded higher carotenoid content (3.73 mg L-1), than cyanobacterial species Microalgal biomass plays a great role as a potential source of natural antioxidants, not only due to the carotenoids but also the phenolic compounds.

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156-159

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September 2014

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[1] O.M.M. Skulberg, Bioactive chemicals in microalgae. In: Richmond A. (ed. ) Handbook of microalgal culture, biotechnology and applied phycology. Oxford: Blackwell; 2006, pp.485-512.

DOI: 10.1002/9780470995280.ch30

Google Scholar

[2] A. Richmond, Handbook of microalge mass culture. Bocaraton, Florida: CRC Press Inc.; (1986).

Google Scholar

[3] M. A. Borowitzka, Algal biotechnology products and processes matching science and economics. J. Appl. Phycol. 1992; 4: 267-279.

DOI: 10.1007/bf02161212

Google Scholar

[4] N.S.A. Pugh, H.N. Ross, M.A. ElSohly, D.S. ElSohly, Isolation of three highmolecular weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, Aphanizomenon flosaquae and Chlorella pyrenoidosa. Planta Med 2001; 67: 737-742.

DOI: 10.1055/s-2001-18358

Google Scholar

[5] S. Takaichi Carotenoids in algae: distributions, biosyntheses and functions. Mar. Drugs 9(2011)1101–1118.

DOI: 10.3390/md9061101

Google Scholar

[6] O. Pulz , W. Gross , Valuable products from biotechnology of microalgae. Appl. Microbial. Biot. 65(2004) 635–648.

DOI: 10.1007/s00253-004-1647-x

Google Scholar

[7] P. Spolaore , C. Joannis-Cassan ,E. Duran , A. Isambert . Commercial applications of microalgae. J. Biosci. Bioeng 101(2006) 87– 96.

DOI: 10.1263/jbb.101.87

Google Scholar

[8] R. Rippka, Isolation and purification of cyanobacteria. Methods Enzymol, 167(1988) 3-27.

Google Scholar

[9] C. Zarrouk, Contribution á l'étude d'une cyanophycée. Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthése de Spirulina maxima (Setch. Et Gardner) Geitler. Ph. D. Thesis. University of Paris, France(1966).

Google Scholar

[10] H.W. Bischoff, H.C. Bold, Trichosarcina polymorphagen. et sp. Nov. J. Phycol. . 1(1963) 34-38.

Google Scholar

[11] C. Yen, H.Y. Chen, Antioxidant Activity Of Various Tea Extracts In Realation To Their Antimutagenecity. J. Agri. Food Chem.., 43 (1995) 27-37.

Google Scholar

[12] G. C. Yen, P. D. Duh, Scavenging Effect Of Methanolic Extracts Of Peanut Hulls On Free-Radical And Active-Oxygen Species. J. Agri. Food Chem. 42 (1994) 629–632.

DOI: 10.1021/jf00039a005

Google Scholar

[13] V. L. Singleton, J. A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Ameri. J. Enology Viticul. 16, (1965) 144–153.

DOI: 10.5344/ajev.1965.16.3.144

Google Scholar

[14] E.M.J. Jaspars, Pigmentation of Tobacco crown-gall tissues cultured In vitro in dependence of the composition of the medium. Physiol. Plant., 18(, (1965): 933-940.

DOI: 10.1111/j.1399-3054.1965.tb06990.x

Google Scholar

[15] R. Tsao, Z. Deng, Separation procedures for naturally occurring antioxidant phytochemicals. J. Chromatogr. 812(2004 ) 85-99.

DOI: 10.1016/s1570-0232(04)00764-0

Google Scholar

[16] G.W. Burton, Antioxidant action of carotenoids. J. Nutr., 119 (1989) 109-111.

Google Scholar

[17] N.I. Krinsky, Antioxidant functions of carotenoids. Free Radic. Biol. Med., 7(1989) 617-635.

Google Scholar

[18] A. Jimenez-Escrig , I. Jimenez-Jimenez , R. Pulido , Saura-Calixto F. Antioxidant activity of fresh and processed edible seaweeds. J. Sci. Food Agric., 81(2001) 530-534.

DOI: 10.1002/jsfa.842

Google Scholar

[19] K. Manivannan, P. Anantharaman, T. Balasubramanian, Evaluation of antioxidant properties of marine microalga Chlorella marina (Butcher, 1952). Asian Pacific J. Trop. Biomed., (2012)S342-S346.

DOI: 10.1016/s2221-1691(12)60185-3

Google Scholar

[20] B. Klejdus , B. LojkovaL,M. Plaza , M. Snoblova, D. Stěrbova , Hyphenated technique for the extraction and determination of isoflavones in algae: Ultrasound-assisted supercritical fluid extraction followed by fast chromatography with tandem mass spectrometry. J. Chromatogr A . 1217(2010).

DOI: 10.1016/j.chroma.2010.07.020

Google Scholar