Abstract: Based on the 3 years observation of Phyllostachys edulis infected by chronic wilt disease, the pathogen was isolated purified and indentified. The result showed that the pathogen of moso bamboo chronic wilt was Gliocladium sp.. Its primary conidiophore was Vertreillium-type braches, 96～128λm tall, phiaides in whorls of 4～5, secondary, pericillate conidiophores,stipes 64～128μm tong,phiaides(6.4～9.6)μm×(4.0～4.8)μm; the dimensions of conidia was (2.4～6.2)μmX (1.9～3.2)#m.