氮素水平对青钱柳叶片主要次生代谢物含量和抗氧化能力的影响

doi:10.3969/j.issn.1000-2006.201904048

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南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (2): 35-42.doi: 10.3969/j.issn.1000-2006.201904048

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氮素水平对青钱柳叶片主要次生代谢物含量和抗氧化能力的影响

岳喜良¹(), 秦健¹, 洑香香¹^,², 尚旭岚¹^,², 方升佐¹^,²^,^*()

1.南京林业大学林学院,江苏南京 210037
2.南京林业大学,南方现代林业协同创新中心,江苏南京 210037

收稿日期:2019-04-21 修回日期:2019-06-24 出版日期:2020-03-30 发布日期:2020-04-01
通讯作者: 方升佐
基金资助:
国家林业局林业科学技术推广项目([2017]08);江苏省自然科学基金项目(BK20160926)

Effects of nitrogen fertilization on secondary metabolite accumulation and antioxidant capacity of Cycolcurya paliurus (Batal.) Iljinskaja leaves

YUE Xiliang¹(), QIN Jian¹, FU Xiangxiang¹^,², SHANG Xulan¹^,², FANG Shengzuo¹^,²^,^*()

1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Received:2019-04-21 Revised:2019-06-24 Online:2020-03-30 Published:2020-04-01
Contact: FANG Shengzuo

摘要/Abstract

摘要：

【目的】通过研究施氮量对青钱柳生长、次生代谢产物积累和抗氧化能力的影响,为青钱柳叶用人工林氮素施肥管理提供理论依据。【方法】以青钱柳两年生平茬实生苗为试验材料,在人工气候室控制条件下,设置N0(0 g/株)、N1(3 g/株)和N2(6 g/株)3水平,氮素施肥水平处理,测定不同处理下青钱柳生长指标,叶全碳、全氮、次生代谢物含量和抗氧化能力,并采用单因素方差分析比较不同氮素处理间的差异。【结果】施氮量对青钱柳苗高、地径、生物量、叶总黄酮、总三萜和总多酚类化合物含量有显著影响(P < 0.05)。随着施氮量的增加,青钱柳苗高、地径和生物量变幅分别为27.33~39.67 cm、6.65~9.19 mm和2.41~3.87 g,均在N1处理下最高;次生代谢物含量在N0处理下最高,但随施氮量的增加,叶中全氮含量显著增加,碳氮比显著降低。相关分析表明,青钱柳叶中全氮含量与碳氮比呈极显著负相关( P < 0.01),ABTS[2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐]和DPPH(1,1-二苯基-2-三硝基苯肼)的自由基半清除浓度(IC ₅₀)值与青钱柳叶中总黄酮、总三萜和总多酚类化合物含量呈显著负相关(P < 0.05)。青钱柳叶总黄酮、总三萜和总多酚类化合物含量及抗氧化能力随施氮量的增加依次表现为N0 > N2 > N1。【结论】施氮量显著影响青钱柳苗高、地径、生物量、叶碳氮比、次生代谢物积累和抗氧化能力。碳氮比与总多酚类化合物含量呈显著正相关,与总黄酮和总三萜类化合物含量及抗氧化能力无相关性。低氮(N0)和高氮(N2)胁迫有利于青钱柳叶中次生代谢物积累和抗氧化能力的提高,但均不利于青钱柳生长。研究结果可为青钱柳叶用林培育的氮肥管理提供理论依据。

关键词: 青钱柳, 施氮量, 次生代谢物, 抗氧化能力, 生长, 全碳, 全氮

Abstract:

【Objective】 Cyclocarya paliurus (Batal.) Iljinskaja, a native species of China, is cultivated for its medicinal properties,fine timber and ornamental value. To form a theoretical framework of nitrogen fertilization management, this study investigated how variable levels of nitrogen influence the growth, accumulation of secondary metabolites and antioxidant capacity of C. paliurus (Batal.) Iljinskaja seedlings.【Method】The temperature and humidity were fixed in the artificial climate chamber, the stumping seedlings of C. paliurus (Batal.) Iljinskaja with two-year-old seedlings were treated by three nitrogen application levels as N1 (0 g/plant), N2 (3 g/plant) and N3 (6 g/plant). And then, the corresponding growth, total carbon, total nitrogen, secondary metabolites and antioxidant capacity of C. paliurus (Batal.) Iljinskaja leaves were measured and compared across treatments by single factor analysis of variance. 【Result】The seedlings height, ground diameter, biomass and secondary metabolite content of C. paliurus (Batal.) Iljinskaja leaves changed significantly across treatments (P < 0.05). With the increase in nitrogen level, the seedlings height, ground diameter and biomass variation of C. paliurus (Batal.) Iljinskaja were 27.33-39.67 cm, 6.65-9.19 mm, and 2.41-3.87 g, respectively; all the highest values were detected with N1 treatment. The highest content of secondary metabolites was observed in N0 treatment. However, the total nitrogen content in seedlings leaves increased with the nitrogen level, and the C/N ratio significantly decreased. In addition, there was a significant negative correlation between the total nitrogen content and C/N ratio in the leaves of C. paliurus (Batal.) Iljinskaja(P < 0.01), as well as between the IC ₅₀ values of ABTS[2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)] and DPPH(1,1-diphenyl-2-picrylhydrazyl) and the contents of total triterpenoids, total flavonoids, and total polyphenols in the leaves ofC. paliurus (Batal.) Iljinskaja(P < 0.05). With increasing nitrogen level, both the secondary metabolite contents measured and their antioxidant capacities in C. paliurus (Batal.) Iljinskaja leaves showed the tendency N0 > N2 > N1. 【Conclusion】The difference in nitrogen level significantly changed the growth, C/N ratio, secondary metabolites accumulation and antioxidant capacity of C. paliurus (Batal.) Iljinskaja seedlings. The C/N ratio had a significant positive correlation with total polyphenols, and no correlation with total flavonoids, total triterpenoids and antioxidant capacity. Both low and high nitrogen treatments contributed to the accumulation of secondary metabolites and an improvement in the antioxidant capacity ofC. paliurus (Batal.) Iljinskaja seedlings, but inhibited the growth of C. paliurus (Batal.) Iljinskaja seedlings. In conclusion, these results provide the theoretical basis for nitrogen fertilization management through C. paliurus (Batal.) Iljinskaja cultivation.

Key words: Cyclocarya paliurus (Batal.) Iljinskaja, nitrogen fertilization, secondary metabolites: antioxidant capacity, growth, total carbon, total nitrogen

中图分类号:

岳喜良,秦健,洑香香,等. 氮素水平对青钱柳叶片主要次生代谢物含量和抗氧化能力的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(2): 35-42.

YUE Xiliang, QIN Jian, FU Xiangxiang, SHANG Xulan, FANG Shengzuo. Effects of nitrogen fertilization on secondary metabolite accumulation and antioxidant capacity of Cycolcurya paliurus (Batal.) Iljinskaja leaves[J].Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(2): 35-42.DOI: 10.3969/j.issn.1000-2006.201904048.

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取样时间/d sampling time	处理 treatments	全C含量/(mg·g^-1) total carbon content	全N含量/(mg·g^-1) total nitrogen content	碳氮比 C/N
	N0	52.20±2.05 Aa	0.75±0.09 Ac	69.72±10.35 Aa
60	N1	50.93±1.37 Aa	0.99±0.13 Bb	51.67±9.22 Ab
	N2	49.36±2.13 Aa	1.54±0.04 Ba	32.05±1.41 Ac
	N0	51.44±0.82 Aa	0.80±0.00 Ac	64.04±0.85 Aa
90	N1	50.79±2.34 Aa	1.39±0.08 Ab	36.53±3.22 Bb
	N2	47.35±1.00 Ab	1.76±0.02 Aa	26.96±0.92 Ab

因子 factors	总三萜含量 TT content	总黄酮含量 TF content	总多酚含量 TPC content	全氮含量 TN content	ABTS的IC₅₀ IC₅₀ of ABTS	DPPH的IC₅₀ IC₅₀ of DPPH
总黄酮含量TF content	0.736^**
总多酚含量TPC content	0.508^*	0.650^**
全氮含量TN content	0.194	0.169	0.443
ABTS的IC₅₀ IC₅₀ of ABTS	-0.914^**	-0.870^**	-0.612^**	-0.104
DPPH的IC₅₀ IC₅₀ of DPPH	-0.865^**	-0.853^**	-0.596^**	-0.080	0.986^**
碳氮比C/N	-0.147	-0.095	0.530*	-0.970^**	0.033	0.018

氮素水平对青钱柳叶片主要次生代谢物含量和抗氧化能力的影响

Effects of nitrogen fertilization on secondary metabolite accumulation and antioxidant capacity of Cycolcurya paliurus (Batal.) Iljinskaja leaves

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审稿

[1]	魏绪英, 张瑶, 马美霞, 姜雪茹, 陈慧婷, 吴靖, 杨玉, 蔡军火. 石蒜年生长周期内NSC及其代谢酶活性变化[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 106-114.
[2]	张曦文, 陈旭, 吴军, 孙国飞, 吴力国, 赵长海, 代伟昭, 刘桂丰. 彩叶桦新品种幼龄期生长适应性早期分析[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 124-130.
[3]	魏静, 谭星, 王昌盛, 闫瑞, 李林珂, 宁月, 刘芸. 引种美国红枫在两种紫色土区的生长和光合特性比较[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 97-105.
[4]	欧阳, 欧阳芳群, 孙猛, 王超, 王军辉, 安三平, 王丽芳, 许娜, 王猛. 欧洲云杉无性系幼龄生长节律、年度和密度互作效应及选择策略[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 95-104.
[5]	王纪, 方升佐. 不同抗褐化剂对青钱柳愈伤组织酶活性和生长的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 167-174.
[6]	梁文超, 步行, 罗思谦, 谢寅峰, 胡加玲, 张往祥. 施肥对增温促花后‘长寿冠’海棠叶片生长及光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 114-120.
[7]	李建新, 徐森, 杨丽婷, 陈双林, 郭子武. 毛竹林下多花黄精构件生物量分配特征的年际效应[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 121-128.
[8]	魏亚娟, 郭靖, 党晓宏, 解云虎, 汪季, 李小乐, 吴慧敏. 吉兰泰荒漠绿洲过渡带不同生境下白刺灌丛沙堆形态特征与影响机制[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 172-180.
[9]	贾瑞瑞, 祝艳艳, 杨秀莲, 付钰, 岳远征, 王良桂. 不同砧木对楸树嫁接苗生长及光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 97-106.
[10]	盖军鹏, 陈东升, 贾炜玮, 王政. 基于种源和气候效应的日本落叶松树高生长模型研究[J]. 南京林业大学学报（自然科学版）, 2023, 47(4): 51-60.
[11]	何潇, 雷相东, 段光爽, 丰庆荣, 张逸如, 冯林艳. 气候变化对落叶松人工林生物量生长的影响模拟[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 120-128.
[12]	袁莹, 王雪峰, 王甜, 陈飞飞, 黄川腾, 林玲, 董晓娜. 基于多图像特征的幼龄沉香全氮估测[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 19-28.
[13]	黄梓良, 徐子恒, 孙操稳. 青钱柳种子雨的季节动态及土壤种子库特征[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 18-26.
[14]	欧建德. 杈干现象对南方红豆杉树冠形态、生长和形质的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 87-94.
[15]	郭常酉, 郭宏仙, 王宝华. 基于气候因子的杉木单木胸径生长模型构建[J]. 南京林业大学学报（自然科学版）, 2023, 47(1): 47-56.