中国农业科学 ›› 2010, Vol. 43 ›› Issue (3): 552-558 .doi: 10.3864/j.issn.0578-1752.2010.03.015

• 园艺 • 上一篇    下一篇

中国主栽银耳配对香灰菌的系统发育和遗传多样性

温文婷,贾定洪,郭勇,孙群,杨志荣,彭卫红

  

  1. (四川大学生命科学学院)

  • 收稿日期:2009-05-05 修回日期:2009-07-10 出版日期:2010-02-10 发布日期:2010-02-10
  • 通讯作者: 彭卫红

Phylogeny and Genetic Diversity of Annulohypoxylon spp. Paired with Cultivated Tremella fuciformis Berk.

WEN Wen-ting, JIA Ding-hong, GUO Yong, SUN Qun, YANG Zhi-rong, PENG Wei-hong
  

  1. (四川大学生命科学学院)

  • Received:2009-05-05 Revised:2009-07-10 Online:2010-02-10 Published:2010-02-10
  • Contact: PENG Wei-hong

摘要:

【目的】了解中国主栽银耳配对香灰菌的分类地位及遗传多样性,为香灰菌资源利用和育种提供分子生物学依据。【方法】采用18S-28S rDNA 间隔区(ITS4/ITS5) 的PCR 扩增和内部简单重复序列多态性(ISSR) 分子标记,对中国主栽银耳配对香灰菌的遗传多样性进行分析。【结果】16 株供试菌株ITS 序列分析表明,供试菌株与Annulohypoxylon stygium 关系最为接近,同源性高达99%。从70 个ISSR引物中筛选出了11 个多态性好 的引物,共获得62 条ISSR标记。以相关系数0.7 为阈值,将16 株菌划为5 个遗传组,菌株间遗传差异较大,遗传多样性较高。【结论】中国主栽银耳地区的香灰菌遗传资源较为丰富,这为利用香灰菌资源选育新品种提供了可能。

关键词: 银耳, 伴生菌, 遗传多样性, 聚类分析

Abstract:

【Objective】 The aim of this paper is to study the classification and genetic diversity of Xianghui and to provide biological information on utilization and breeding of Xianghui. 【Method】 The phylogeny of 16 strains of Xianghui, which were collected from different provinces and paired with cultivated Tremella, was determined by ITS sequences analysis. Furthermore, the genetic diversity of these strains was analyzed by inter-simple sequence repeat (ISSR). 【Result】 The ITS sequences of the 16 strains were analyzed and the phylogenic tree was constructed, and the results revealed that the homology between Xianghui and Annulohypoxylon stygium was 99%. Among the 70 ISSR primers, 11 with high polymorphism were selected and a total of 62 ISSR markers were obtained. At 0.7 similarity distance, cluster analysis revealed the existence of five distinct genetic groups. 【Conclusion】 The 16 strains of Annulohypoxylon spp. investigated showed low genetic similarity and genetic variations at DNA level significantly.

Key words: Tremella fuciformis, accompanying fungi, genetic diversity, cluster analysis