Abstract
Scutellariae radix, the root of Scutellaria baiealensis, is a traditional medicinal herb containing baicalin and wogonoside as the main flavonoids. Before absorption, these flavonoids are metabolized into their corresponding aglycones, baicalein and wogonin, by β-glucuronidase produced by the intestinal microflora. To develop a method for efficient transformation of these flavonoids, optimal conditions for the production of β-glucuronidase from Lactobacillus delbrueckii Rh2 (Rh2) were determined. Addition of 4% galactose to basal medium increased the enzyme activity of Rh2 by about 8-fold. Optimal pH and temperature for the β-glucuronidase activity of cell extract were 5.0 and 50°C, respectively, and more than 95% of the enzyme activity was maintained at 60°C for 11 h. Under optimal conditions, more than 90% of the glycones were converted into their corresponding aglycones within 3 h. These results demonstrate that the transformation of baicalin and wogonoside from S. baicalensis by Rh2 would be useful for the efficient production of wogonin and baicalein.
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Ku, S., Zheng, H., Park, M.S. et al. Optimization of β-glucuronidase activity from Lactobacillus delbrueckii Rh2 and and its use for biotransformation of baicalin and wogonoside. J. Korean Soc. Appl. Biol. Chem. 54, 275–280 (2011). https://doi.org/10.3839/jksabc.2011.043
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DOI: https://doi.org/10.3839/jksabc.2011.043